Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing, 210044, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.
Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing, 210044, China.
Environ Pollut. 2022 Oct 15;311:119932. doi: 10.1016/j.envpol.2022.119932. Epub 2022 Aug 13.
To better promote environment friendly development of the coal chemical industry, this study investigated effects of methanol, sodium citrate, and chlorella powder (a type of microalgae) as co-metabolic substances on enhanced anaerobic treatment of coal pyrolysis wastewater with anaerobic sludge. The anaerobic sludge was loaded into four 2 L anaerobic reactors for co-metabolism enhanced anaerobic experiments. Anaerobic reactor 1 (R1) as control group did not add a co-metabolic substance; anaerobic reactor 2 (R2) added methanol; anaerobic reactor 3 (R3) added sodium citrate; and anaerobic reactor 4 (R4) added chlorella powder. In the blank control group, the removal ratios of total phenol (TPh), quinoline, and indole were only 12.07%, 42.15%, and 50.47%, respectively, indicating that 50 mg/L quinoline, 50 mg/L indole, and 600 mg/L TPh produced strong toxicity inhibition function on the anaerobic microorganism in reactor. When the concentration of methanol, sodium citrate, and chlorella was 400 μg/L, the reactors with co-metabolic substances had better treatment effect on TPh. Among them, the strengthening effects of sodium citrate (TPh removal ratio: 44.87%) and chlorella (47.85%) were better than that of methanol (38.72%) and the control group (10.62%). Additionally, the reactors with co-metabolic substances had higher degradation ratios on quinoline, indole, and chemical oxygen demand (COD). The data of extracellular polymeric substances showed that with the co-metabolic substances, anaerobic microorganisms produced more humic acids by degrading phenols and nitrogen-containing heterocyclic compounds (NHCs). Compared with the control group, the reactors added with sodium citrate and chlorella had larger average particle size of sludge. Thus, sodium citrate and chlorella could improve sludge sedimentation performance by increasing the sludge particle size. The bacterial community structures of reactors were explored and the results showed that Aminicenantes genera incertae sedis, Levinea, Geobacter, Smithella, Brachymonas, and Longilinea were the main functional bacteria in reactor added with chlorella.
为了更好地促进煤化工的环境友好发展，本研究考察了甲醇、柠檬酸钠和小球藻粉（一种微藻）作为共代谢物质对厌氧污泥强化处理煤热解废水的影响。将厌氧污泥装入四个 2L 厌氧反应器中进行共代谢强化厌氧实验。厌氧反应器 1（R1）作为对照组，不添加共代谢物质；厌氧反应器 2（R2）添加甲醇；厌氧反应器 3（R3）添加柠檬酸钠；厌氧反应器 4（R4）添加小球藻粉。在空白对照组中，总酚（TPh）、喹啉和吲哚的去除率仅为 12.07%、42.15%和 50.47%，表明 50mg/L 喹啉、50mg/L 吲哚和 600mg/L TPh 对反应器中厌氧微生物产生了强烈的毒性抑制作用。当甲醇、柠檬酸钠和小球藻的浓度分别为 400μg/L 时，含共代谢物质的反应器对 TPh 的处理效果较好。其中，柠檬酸钠（TPh 去除率：44.87%）和小球藻（47.85%）的强化效果优于甲醇（38.72%）和对照组（10.62%）。此外，含共代谢物质的反应器对喹啉、吲哚和化学需氧量（COD）的降解率更高。胞外聚合物的数据分析表明，在共代谢物质的作用下，厌氧微生物通过降解酚类和含氮杂环化合物（NHCs）产生了更多的腐殖酸。与对照组相比，添加柠檬酸钠和小球藻的反应器的污泥平均粒径更大。因此，柠檬酸钠和小球藻可以通过增加污泥粒径来改善污泥沉降性能。还对反应器中的细菌群落结构进行了探索，结果表明，小球藻添加的反应器中的主要功能细菌为 Aminicenantes 属未确定种、Levinea、Geobacter、Smithella、Brachymonas 和 Longilinea。
