The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, 510900, China.
Department of Medical Laboratory, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong, 510515, China.
Nat Commun. 2022 Aug 16;13(1):4804. doi: 10.1038/s41467-022-32505-8.
Metabolite alteration has been associated with the pathogenesis of inflammatory bowel disease (IBD), including colitis. Mannose, a natural bioactive monosaccharide that is involved in metabolism and synthesis of glycoproteins, exhibits anti-inflammatory and anti-oxidative activities. We show here that the circulating level of mannose is increased in patients with IBD and mice with experimental colitis. Mannose treatment attenuates intestinal barrier damage in two mouse colitis models, dextran sodium sulfate (DSS)-induced colitis and spontaneous colitis in IL-10-deficient mice. We demonstrate that mannose treatment enhanced lysosomal integrity and limited the release of cathepsin B, preventing mitochondrial dysfunction and myosin light chain kinase (MLCK)-induced tight junction disruption in the context of intestinal epithelial damage. Mannose exerts a synergistic therapeutic effect with mesalamine on mouse colitis. Cumulatively, the results indicate that mannose supplementation may be an optional approach to the treatment of colitis and other diseases associated with intestinal barrier dysfunction.
代谢物的改变与炎症性肠病（IBD）的发病机制有关，包括结肠炎。甘露糖是一种天然的生物活性单糖，参与糖蛋白的代谢和合成，具有抗炎和抗氧化作用。我们在这里表明，甘露糖的循环水平在 IBD 患者和实验性结肠炎小鼠中增加。甘露糖治疗可减轻两种小鼠结肠炎模型（葡聚糖硫酸钠（DSS）诱导的结肠炎和 IL-10 缺陷型小鼠自发结肠炎）中的肠道屏障损伤。我们证明甘露糖治疗增强了溶酶体的完整性，并限制了组织蛋白酶 B 的释放，防止了线粒体功能障碍和肌球蛋白轻链激酶（MLCK）诱导的紧密连接在肠上皮损伤情况下的破坏。甘露糖与美沙拉嗪对小鼠结肠炎具有协同治疗作用。总之，这些结果表明，甘露糖的补充可能是治疗结肠炎和其他与肠道屏障功能障碍相关疾病的一种可选方法。
