Byers S, Hopkins T J, Kuettner K E, Kimura J H
J Biol Chem. 1987 Jul 5;262(19):9166-74.
A range of structurally related zwitterionic detergents, Zwittergents 3-06, 3-08, 3-10, and 3-12, and a derivative of cholic acid (Chaps) were examined for their ability to enhance the extraction of newly synthesized, intracellular proteoglycans and for their effect on the functional properties of cartilage proteoglycan. Although none of the detergents could extract greater than 4% of the intracellular proteoglycans when used alone, Zwittergents 3-10, 3-12, and Chaps proved equally as effective when used in combination with 4 M guanidine HCl extracting greater than 90% of newly synthesized proteoglycans. Rate zonal centrifugation of aggregates containing either 3H-link protein or 3H-monomer, which had been incubated with 2% (w/v) detergent indicated that none of the test detergents caused a disassembly of intact aggregates. However, both Zwittergents 3-10 and 3-12 prevented the reaggregation of components dissociated with 4 M guanidine HCl. Similar to the finding with aggregate, none of the detergents caused a disassembly of monomer-link protein complexes prepared from purified 3H-link protein and proteoglycan monomer, while Zwittergents 3-10 and 3-12 prevented their assembly from free link protein and monomer. However, monomer-link protein complexes once formed were able to associate with hyaluronic acid to form link-stable ternary complexes in the presence of all detergents tested including Zwittergents 3-10 and 3-12.
研究了一系列结构相关的两性离子去污剂（两性离子去污剂3-06、3-08、3-10和3-12）以及胆酸衍生物（CHAPS）促进新合成的细胞内蛋白聚糖提取的能力及其对软骨蛋白聚糖功能特性的影响。尽管单独使用时，这些去污剂均不能提取超过4%的细胞内蛋白聚糖，但两性离子去污剂3-10、3-12和CHAPS与4M盐酸胍联合使用时同样有效，能提取超过90%的新合成蛋白聚糖。对含有3H连接蛋白或3H单体的聚集体进行速率区带离心，这些聚集体已与2%（w/v）去污剂孵育，结果表明，受试去污剂均未导致完整聚集体的解离。然而，两性离子去污剂3-10和3-12均能阻止与4M盐酸胍解离的组分重新聚集。与聚集体的研究结果相似，这些去污剂均未导致由纯化的3H连接蛋白和蛋白聚糖单体制备的单体-连接蛋白复合物解离，而两性离子去污剂3-10和3-12则阻止了它们由游离连接蛋白和单体组装形成。然而，一旦形成，单体-连接蛋白复合物能够在包括两性离子去污剂3-10和3-12在内的所有受试去污剂存在的情况下与透明质酸结合形成连接稳定的三元复合物。
