软骨蛋白聚糖聚集体的形成。连接蛋白的作用。
Cartilage proteoglycan aggregate formation. Role of link protein.
作者信息
Franzén A, Björnsson S, Heinegård D
出版信息
Biochem J. 1981 Sep 1;197(3):669-74. doi: 10.1042/bj1970669.
Abstract
Cartilage proteoglycan aggregate formation was studied by zonal rate centrifugation in sucrose gradients. Proteoglycan aggregates, monomers and proteins could be resolved. It was shown that the optimal proportion of hyaluronic acid for proteoglycan aggregate formation was about 1% of proteoglycan dry weight. The reaggregation of dissociated proteoglycan aggregate A1 fraction was markedly concentration-dependent and even at 9 mg/ml only about 90% of the aggregates were reformed. The lowest proportion of link protein required for maximal formation of link-stabilized proteoglycan aggregates was 1.5% of proteoglycan dry weight. It was separately shown that link protein co-sedimented with the proteoglycan monomer. By competition with isolated hyaluronic acid-binding-region fragments, a proportion of the link proteins was removed from the proteoglycan monomers, indicating that the link protein binds to the hyaluronic acid-binding region of the proteoglycan monomer.
摘要
通过在蔗糖梯度中进行区带速率离心研究软骨蛋白聚糖聚集体的形成。蛋白聚糖聚集体、单体和蛋白质可以被分离。结果表明，蛋白聚糖聚集体形成的透明质酸最佳比例约为蛋白聚糖干重的1%。解离的蛋白聚糖聚集体A1组分的重新聚集明显依赖于浓度，即使在9mg/ml时，也只有约90%的聚集体重新形成。形成连接稳定的蛋白聚糖聚集体所需的连接蛋白的最低比例为蛋白聚糖干重的1.5%。另外还表明，连接蛋白与蛋白聚糖单体共同沉降。通过与分离的透明质酸结合区域片段竞争，一部分连接蛋白从蛋白聚糖单体中被去除，这表明连接蛋白与蛋白聚糖单体的透明质酸结合区域结合。
相似文献
1
Cartilage proteoglycan aggregate formation. Role of link protein.软骨蛋白聚糖聚集体的形成。连接蛋白的作用。
Biochem J. 1981 Sep 1;197(3):669-74. doi: 10.1042/bj1970669.
2
Assembly of proteoglycan aggregates in cultures of chondrocytes from bovine tracheal cartilage.牛气管软骨软骨细胞培养物中蛋白聚糖聚集体的组装
Biochem J. 1981 Oct 1;199(1):17-29. doi: 10.1042/bj1990017.
3
A study of the interaction between cartilage proteoglycan and link protein.软骨蛋白聚糖与连接蛋白之间相互作用的研究。
Biochem J. 1987 Dec 15;248(3):943-51. doi: 10.1042/bj2480943.
4
The effect of link protein on proteoglycan aggregate structure. An electron microscopic study of the molecular architecture and dimensions of proteoglycan aggregates reassembled from the proteoglycan monomers and link proteins of bovine fetal epiphyseal cartilage.连接蛋白对蛋白聚糖聚集体结构的影响。对从牛胎儿骺软骨的蛋白聚糖单体和连接蛋白重新组装的蛋白聚糖聚集体的分子结构和尺寸进行的电子显微镜研究。
J Biol Chem. 1984 May 10;259(9):5361-3.
5
Assembly of newly synthesized proteoglycan and link protein into aggregates in cultures of chondrosarcoma chondrocytes.在软骨肉瘤软骨细胞培养物中，新合成的蛋白聚糖和连接蛋白组装成聚集体。
J Biol Chem. 1980 Aug 10;255(15):7134-43.
6
7
Proteoglycan aggregate formation by articular chondrocytes. Decrease in link-protein synthesis during culture.关节软骨细胞形成蛋白聚糖聚集体。培养过程中连接蛋白合成减少。
Biochem J. 1983 Sep 15;214(3):855-64. doi: 10.1042/bj2140855.
8
Degradation of human proteoglycan aggregate induced by hydrogen peroxide. Protein fragmentation, amino acid modification and hyaluronic acid cleavage.过氧化氢诱导人蛋白聚糖聚集体的降解。蛋白质片段化、氨基酸修饰及透明质酸裂解。
Biochem J. 1989 May 1;259(3):805-11. doi: 10.1042/bj2590805.
9
10
Evidence of proteoglycan/proteoglycan interactions within aggregates.聚集体内蛋白聚糖/蛋白聚糖相互作用的证据。
J Orthop Res. 1991 Nov;9(6):777-86. doi: 10.1002/jor.1100090602.
引用本文的文献
1
Activin A suppresses osteoblast mineralization capacity by altering extracellular matrix (ECM) composition and impairing matrix vesicle (MV) production.激活素 A 通过改变细胞外基质 (ECM) 组成和损害基质小泡 (MV) 产生来抑制成骨细胞的矿化能力。
Mol Cell Proteomics. 2013 Oct;12(10):2890-900. doi: 10.1074/mcp.M112.024927. Epub 2013 Jun 17.
2
Harnessing cell–biomaterial interactions for osteochondral tissue regeneration.利用细胞-生物材料相互作用进行骨软骨组织再生。
Adv Biochem Eng Biotechnol. 2012;126:67-104. doi: 10.1007/10_2011_107.
3
Periovulatory expression of hyaluronan and proteoglycan link protein 1 (Hapln1) in the rat ovary: hormonal regulation and potential function.大鼠卵巢中透明质酸和蛋白聚糖连接蛋白1（Hapln1）在围排卵期的表达：激素调节及潜在功能
Mol Endocrinol. 2010 Jun;24(6):1203-17. doi: 10.1210/me.2009-0325. Epub 2010 Mar 25.
4
Cartilage stress-relaxation is affected by both the charge concentration and valence of solution cations.软骨应力松弛受溶液阳离子的电荷浓度和价态影响。
Osteoarthritis Cartilage. 2009 May;17(5):669-76. doi: 10.1016/j.joca.2008.09.011. Epub 2008 Oct 10.
5
Hyaluronan-dependent pericellular matrix.透明质酸依赖性细胞周基质
Adv Drug Deliv Rev. 2007 Nov 10;59(13):1351-65. doi: 10.1016/j.addr.2007.08.008. Epub 2007 Aug 14.
6
Distribution of hyaluronan in articular cartilage as probed by a biotinylated binding region of aggrecan.通过聚集蛋白聚糖的生物素化结合区域探测透明质酸在关节软骨中的分布。
Histochem Cell Biol. 1996 Mar;105(3):187-94. doi: 10.1007/BF01462291.
7
The expression of functional link protein in a baculovirus system: analysis of mutants lacking the A, B and B' domains.杆状病毒系统中功能连接蛋白的表达：对缺失A、B和B'结构域的突变体的分析
Biochem J. 1994 Jun 1;300 ( Pt 2)(Pt 2):317-24. doi: 10.1042/bj3000317.
8
Evidence of a defined spatial arrangement of hyaluronate in the central filament of cartilage proteoglycan aggregates.软骨蛋白聚糖聚集体中央细丝中透明质酸盐特定空间排列的证据。
Biochem J. 1995 Apr 15;307 ( Pt 2)(Pt 2):595-601. doi: 10.1042/bj3070595.
9
Assembly of proteoglycan aggregates in cultures of chondrocytes from bovine tracheal cartilage.牛气管软骨软骨细胞培养物中蛋白聚糖聚集体的组装
Biochem J. 1981 Oct 1;199(1):17-29. doi: 10.1042/bj1990017.
10
Metabolism of cartilage proteins in cultured tissue sections.培养组织切片中软骨蛋白的代谢
Biochem J. 1983 Jun 15;212(3):659-67. doi: 10.1042/bj2120659.
本文引用的文献
1
Equilibrium-binding studies of pig laryngeal cartilage proteoglycans with hyaluronate oligosaccharide fractions.猪喉软骨蛋白聚糖与透明质酸寡糖组分的平衡结合研究。
Biochem J. 1980 Jan 1;185(1):107-14. doi: 10.1042/bj1850107.
2
Immunochemical analysis of cartilage proteoglycans. Radioimmunoassay of the molecules and the substructures.软骨蛋白聚糖的免疫化学分析。分子及亚结构的放射免疫测定。
Biochem J. 1980 Jun 1;187(3):687-94. doi: 10.1042/bj1870687.
3
Proteoglycans from bovine nasal cartilage. Immunochemical studies of link protein.来自牛鼻软骨的蛋白聚糖。连接蛋白的免疫化学研究。
J Biol Chem. 1980 Oct 10;255(19):9295-305.
4
Characterization of chondroitin sulfate isolated from trypsin-chymotrypsin digests of cartilage proteoglycans.从软骨蛋白聚糖的胰蛋白酶-糜蛋白酶消化物中分离得到的硫酸软骨素的特性分析。
Arch Biochem Biophys. 1974 Nov;165(1):427-41. doi: 10.1016/0003-9861(74)90182-9.
5
Aggregation of cartilage proteoglycans. 3. Characteristics of the proteins isolated from trypsin digests of aggregates.软骨蛋白聚糖的聚集。3. 从聚集物胰蛋白酶消化物中分离出的蛋白质的特性。
J Biol Chem. 1974 Jul 10;249(13):4250-6.
6
Aggregation of cartilage proteoglycans. II. Oligosaccharide competitors of the proteoglycan-hyaluronic acid interaction.软骨蛋白聚糖的聚集。II. 蛋白聚糖-透明质酸相互作用的寡糖竞争者。
J Biol Chem. 1974 Jul 10;249(13):4242-9.
7
Aggregation of cartilage proteoglycans. I. The role of hyaluronic acid.软骨蛋白聚糖的聚集。I. 透明质酸的作用。
J Biol Chem. 1974 Jul 10;249(13):4232-41.
8
Extraction, fractionation and characterization of proteoglycans from bovine tracheal cartilage.从牛气管软骨中提取、分离和鉴定蛋白聚糖
Biochim Biophys Acta. 1972 Nov 28;285(1):181-92. doi: 10.1016/0005-2795(72)90190-0.
9
The specific interaction of hyaluronic acid with cartillage proteoglycans.透明质酸与软骨蛋白聚糖的特异性相互作用。
Biochim Biophys Acta. 1972 Sep 15;279(2):401-5. doi: 10.1016/0304-4165(72)90160-2.
Zotero 插件