循环miR-132、miR-146a、miR-222和miR-320表达在多囊卵巢综合征女性鉴别诊断中的作用
CIRCULATING MIR-132, MIR-146A, MIR-222, AND MIR-320 EXPRESSION IN DIFFERENTIAL DIAGNOSIS OF WOMEN WITH POLYCYSTIC OVARY SYNDROME.
作者信息
Soyman Z, Durmus S, Ates S, Simsek G, Sozer V, Kundaktepe B P, Kurtulus D, Gelisgen R, Sal V, Uzun H
机构信息
Istanbul Education and Research Hospital, Dept. of Obstetrics & Gynecology, Istanbul, Turkey.
Istanbul University-Cerrahpasa, Cerrahpasa School of Medicine, Dept. of Biochemistry, Istanbul, Turkey.
出版信息
Acta Endocrinol (Buchar). 2022 Jan-Mar;18(1):13-19. doi: 10.4183/aeb.2022.13.
PURPOSE
The aim of the study was to investigate whether the circulating miR-132, miR-146a, miR-222, and miR-320 levels are used in the differential diagnosis of women with polycystic ovary syndrome (PCOS) and healthy women.
METHODS
This prospective case-control study included 50 women with PCOS and age- and body mass index- matched 50 healthy controls. The hormone and lipid profiles, levels of microRNAs (miRNAs), and parameters of carbohydrate metabolism were measured.
RESULTS
Expression levels of miRNAs were assessed using the two-step quantitative real-time polymerase chain reaction. Circulating miR-132, miR-146a and miR-222 levels were significantly downregulated in the PCOS group compared with the control group. The miR-320 levels did not differ between the two groups. Free testosterone was negatively correlated with miR-132, miR-146a and miR-222. Insulin was negatively correlated with miR-132 and miR-146a.
CONCLUSIONS
The results of the study revealed that miRNA expression, may suggest a possible distinction between healthy women and PCOS patients. miR-132, miR-146a, and miR-222 may have key functions in the pathogenesis of PCOS.
目的
本研究旨在探讨循环中的miR-132、miR-146a、miR-222和miR-320水平是否可用于多囊卵巢综合征（PCOS）女性与健康女性的鉴别诊断。
方法
这项前瞻性病例对照研究纳入了50例PCOS女性以及50例年龄和体重指数相匹配的健康对照。测量了激素和血脂谱、微小RNA（miRNA）水平以及碳水化合物代谢参数。
结果
采用两步定量实时聚合酶链反应评估miRNA的表达水平。与对照组相比，PCOS组循环中的miR-132、miR-146a和miR-222水平显著下调。两组间miR-320水平无差异。游离睾酮与miR-132、miR-146a和miR-222呈负相关。胰岛素与miR-132和miR-146a呈负相关。
结论
研究结果表明，miRNA表达可能提示健康女性与PCOS患者之间的一种可能区别。miR-132、miR-146a和miR-222可能在PCOS发病机制中起关键作用。
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