Zaytseva Yulianna, Petruk Anastasia, Novikova Tatyana
Central Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences, st. Zolotodolinskaya, 101, Novosibirsk, 630090 Russian Federation.
J Plant Growth Regul. 2023;42(5):2933-2942. doi: 10.1007/s00344-022-10757-4. Epub 2022 Aug 12.
Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B- 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in microshoots.
Turcz.分布于东亚北部地区，一直被认为是黄杉素（二氢槲皮素）和芦丁的替代天然来源。本研究基于生物技术方法开展，旨在开发一种环境友好且高效的系统，以生产黄杉素和芦丁微枝，采用不同浓度的噻苯隆（TDZ）处理（0.1；0.5；2.5 μM）并结合各种类型的光照，包括荧光灯（FL）和发光二极管（LED）（红/蓝 - 80% 红光 + 20% 蓝光；5LED - 20% 红光 + 20% 蓝光 + 20% 绿光 + 20% 黄光 + 20% 白光）。与荧光灯照明相比，在0.5 μM TDZ与5LED组合条件下，每个外植体获得的芽数最多。在不同光照类型和TDZ浓度下获得的芽簇中，在添加2.5 μM TDZ的FL培养基上培养以及在R/B或5LED条件下添加0.5 μM TDZ培养的芽簇，其鲜重和干重均有显著增加。在FL光照下，无TDZ培养基上微枝中的总叶绿素含量增加，而TDZ处理降低了FL和5LED条件下的叶绿素浓度。在5LED条件下使用0.1 μM TDZ降低了叶绿素a + b与类胡萝卜素的比率，并导致黄杉素、芦丁、槲皮素、金丝桃苷和扁蓄苷的积累量最高。因此，已证明LED和TDZ的联合作用在微枝的繁殖效率、生物量积累以及黄杉素和芦丁生产方面具有巨大潜力。
