Key Laboratory of Advanced Design and Intelligent Computing, Ministry of Education, School of Software Engineering, Dalian University, Dalian 116622, China.
School of Computer Science and Technology, Dalian University of Technology, Dalian 116024, China.
Biomolecules. 2023 Mar 5;13(3):481. doi: 10.3390/biom13030481.
The construction of DNA reaction networks with complex functions using various methods has been an important research topic in recent years. Whether the DNA reaction network can perform complex tasks and be recycled directly affects the performance of the reaction network. Therefore, it is very important to design and implement a DNA reaction network capable of multiple tasks and reversible regulation. In this paper, the hairpin allosteric method was used to complete the assembly task of different functional nucleic acids. In addition, information conversion of the network was realized. In this network, multiple hairpins were assembled into nucleic acid structures with different functions to achieve different output information through the cyclic use of trigger strands. A method of single-input dual-output information conversion was proposed. Finally, the network with signal amplification and reversible regulation was constructed. In this study, the reversible regulation of different functional nucleic acids in the same network was realized, which shows the potential of this network in terms of programmability and provides new ideas for constructing complex and multifunctional DNA reaction networks.
近年来，使用各种方法构建具有复杂功能的 DNA 反应网络一直是一个重要的研究课题。DNA 反应网络是否能够执行复杂任务并直接回收，直接影响反应网络的性能。因此，设计和实现能够进行多项任务和可逆调控的 DNA 反应网络非常重要。在本文中，我们使用发夹变构的方法完成了不同功能的核酸的组装任务。此外，我们还实现了网络的信息转换。在这个网络中，多个发夹通过循环使用触发链组装成具有不同功能的核酸结构，从而通过不同的触发链实现不同的输出信息。提出了一种单输入双输出的信息转换方法。最后，构建了具有信号放大和可逆调控功能的网络。在这项研究中，我们在同一个网络中实现了不同功能的核酸的可逆调控，这表明该网络在可编程性方面具有潜力，为构建复杂多功能的 DNA 反应网络提供了新的思路。
