Guangdong Provincial Key Laboratory of Applied Botany and Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Guangzhou, China.
Physiol Plant. 2022 Sep;174(5):e13764. doi: 10.1111/ppl.13764.
Some members of the CYP51G subfamily has been shown to be obtusifoliol 14α-demethylase, key enzyme of the sterol and brassinosteroid (BR) biosynthesis, which mediate plant development and response to stresses. However, little is known about the functions of CYP51H subfamily in rice. Here, OsCYP51H3, an ortholog of rice OsCYP51G1 was identified. Compared with wild type, the mutants oscyp51H3 and OsCYP51H3-RNAi showed dwarf phenotype, late flowering, erected leaves, lower seed-setting rate, and smaller and shorter seeds. In contrast, the phenotypic changes of OsCYP51H3-OE plants are not obvious. Metabolomic analysis of oscyp51H3 mutant indicated that OsCYP51H3 may also encode an obtusifoliol 14α-demethylase involved in phytosterol and BR biosynthesis, but possibly not that of triterpenes. The RNA-seq results showed that OsCYP51H3 may affect the expression of a lot of genes related to rice development. These findings showed that OsCYP51H3 codes for a putative obtusifoliol 14α-demethylase involved in phytosterol and BR biosynthesis, and mediates rice development.
一些 CYP51G 亚家族成员已被证明是角鲨烯 14α-脱甲基酶，这是甾醇和油菜素内酯（BR）生物合成的关键酶，可介导植物发育和对胁迫的反应。然而，对于水稻 CYP51H 亚家族的功能知之甚少。在这里，鉴定了水稻 OsCYP51G1 的同源物 OsCYP51H3。与野生型相比，突变体 oscyp51H3 和 OsCYP51H3-RNAi 表现出矮化表型、晚花、直立叶片、较低的结实率以及更小和更短的种子。相比之下，OsCYP51H3-OE 植物的表型变化不明显。oscyp51H3 突变体的代谢组学分析表明，OsCYP51H3 可能还编码一种参与植物甾醇和 BR 生物合成的角鲨烯 14α-脱甲基酶，但可能不是三萜类化合物。RNA-seq 结果表明，OsCYP51H3 可能影响与水稻发育相关的许多基因的表达。这些发现表明，OsCYP51H3 编码一种参与植物甾醇和 BR 生物合成的假定角鲨烯 14α-脱甲基酶，并介导水稻发育。
