Herrera-Isidron Lisset, Valencia-Lozano Eliana, Uribe-Lopez Braulio, Délano-Frier John Paul, Barraza Aarón, Cabrera-Ponce José Luis
Unidad Profesional Interdisciplinaria de Ingeniería Campus Guanajuato (UPIIG), Instituto Politécnico Nacional, Av. Mineral de Valenciana 200, Puerto Interior, Silao de la Victoria 36275, Guanajuato, Mexico.
Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato 36824, Guanajuato, Mexico.
Plants (Basel). 2024 Aug 27;13(17):2391. doi: 10.3390/plants13172391.
Potato tubers are reproductive and storage organs, enabling their survival. Unraveling the molecular mechanisms that regulate tuberization is crucial for understanding how potatorespond to environmental stress situations and for potato breeding. Previously, we did a transcriptomic analysis of potato microtuberization without light. This showed that important cellular processes like ribosomal proteins, cell cycle, carbon metabolism, oxidative stress, fatty acids, and phytosterols (PS) biosynthesis were closely connected in a protein-protein interaction (PPI) network. Research on PS function during potato tuberization has been scarce. PS plays a critical role in regulating membrane permeability and fluidity, and they are biosynthetic precursors of brassinosteroids (BRs) in plants, which are critical in regulating gene expression, cell division, differentiation, and reproductive biology. Within a PPI network, we found a module of 15 genes involved in the PS biosynthetic process. Darkness, as expected, activated the mevalonate (MVA) pathway. There was a tight interaction between three coding gene products for HMGR3, MVD2, and FPS1, and the gene products that synthetize PS, including CAS1, SMO1, BETAHSD, CPI1, CYP51, FACKEL, HYDRA1, SMT2, SMO2, STE1, and SSR1. Quantitative real-time polymerase chain reaction (qRT-PCR) confirmed the expression analysis of ten specific genes involved in the biosynthesis of PS. This manuscript discusses the potential role of genes involved in PS biosynthesis during microtuber development.
马铃薯块茎是繁殖和储存器官，使其能够存活。阐明调控块茎形成的分子机制对于理解马铃薯如何应对环境胁迫情况以及马铃薯育种至关重要。此前，我们对无光条件下马铃薯微型块茎形成进行了转录组分析。结果表明，核糖体蛋白、细胞周期、碳代谢、氧化应激、脂肪酸和植物甾醇（PS）生物合成等重要细胞过程在蛋白质-蛋白质相互作用（PPI）网络中紧密相连。关于PS在马铃薯块茎形成过程中的功能研究较少。PS在调节膜通透性和流动性方面起关键作用，它们是植物中油菜素内酯（BRs）的生物合成前体，而BRs在调节基因表达、细胞分裂、分化和生殖生物学方面至关重要。在一个PPI网络中，我们发现了一个由15个参与PS生物合成过程的基因组成的模块。正如预期的那样，黑暗激活了甲羟戊酸（MVA）途径。HMGR3、MVD2和FPS1的三个编码基因产物与合成PS的基因产物之间存在紧密相互作用，合成PS的基因产物包括CAS1、SMO1、BETAHSD、CPI1、CYP51、FACKEL、HYDRA1、SMT2、SMO2、STE1和SSR1。定量实时聚合酶链反应（qRT-PCR）证实了参与PS生物合成的10个特定基因的表达分析。本文讨论了参与PS生物合成的基因在微型块茎发育过程中的潜在作用。
