Angello J C, Pendergrass W R, Norwood T H, Prothero J
J Cell Physiol. 1987 Jul;132(1):125-30. doi: 10.1002/jcp.1041320117.
Human foreskin fibroblast-like cells were separated on the basis of DNA content and cell size by fluorescence-activated cell sorting. Subpopulations of "large" or "small" cells with the same (G1) DNA content were clonally expanded and found to contain predominantly nondividing or highly proliferative cells, respectively. From the rate of clonal growth, we deduce that small cells divide faster than large cells. Intermediate-sized cells were found to yield primarily smaller ("attenuated") clones. The clonal data can be incorporated into a previously reported kinetic model of clonal attenuation. This version of the model postulates that small "stem" cells yield larger daughters which have only a limited proliferative potential. We also postulate that a progressive increase in cell size can account for the decreasing concentration of DNA polymerase alpha, which has been reported in older cultures.
通过荧光激活细胞分选，根据DNA含量和细胞大小分离出人包皮成纤维细胞样细胞。对具有相同（G1）DNA含量的“大”或“小”细胞亚群进行克隆扩增，发现分别主要包含不分裂或高增殖细胞。根据克隆生长速率，我们推断小细胞比大细胞分裂得更快。发现中等大小的细胞主要产生较小的（“衰减的”）克隆。这些克隆数据可以纳入先前报道的克隆衰减动力学模型。该模型版本假设小的“干细胞”产生较大的子代细胞，而这些子代细胞的增殖潜力有限。我们还假设细胞大小的逐渐增加可以解释在较老培养物中报道的DNA聚合酶α浓度的降低。
