The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
School of Materials and Energy & Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing, Southwest University, Chongqing, 400715, China.
Biomater Sci. 2022 Sep 27;10(19):5583-5595. doi: 10.1039/d2bm01099b.
Periodontitis is a prevalent chronic inflammatory disease that destroys the periodontal supporting tissues, impinges on oral health, and is correlated with an increased risk of systemic disease. Currently, the main drug treatment is antibiotic therapy; however, systemic antibiotic therapy still has various drawbacks such as bacterial resistance, low bioavailability and burst release. It is noteworthy that the local use of non-antibiotic drugs with sustained release characteristics can effectively overcome these problems. It has been documented that chlorogenic acid (CGA) has good anti-inflammatory and antioxidant properties. To achieve the sustained release of CGA, we synthesized CGA-PLGA@PVP nanomicelles by loading CGA onto poly(D,L-lactide--glycolide) (PLGA) and modified them with polyvinylpyrrolidone (PVP) for better dispersion. The results demonstrated that CGA-PLGA@PVP nanomicelles could prolong the release time of CGA, and could not only effectively remove reactive oxygen species (ROS) but also downregulate the overexpression of proinflammatory cytokines in lipopolysaccharide (LPS)-treated RAW264.7 cells. Moreover, CGA-PLGA@PVP nanomicelles could remain in gingival tissue for more than 24 hours after local injection, inhibit alveolar bone resorption and prevent the progression of periodontitis in a mouse model, showing good biocompatibility. Therefore, CGA-PLGA@PVP nanomicelles have great properties and are expected to be a novel therapeutic strategy for periodontitis.
牙周炎是一种常见的慢性炎症性疾病，会破坏牙周支持组织，影响口腔健康，并与系统性疾病风险增加相关。目前，主要的药物治疗是抗生素治疗；然而，全身抗生素治疗仍然存在细菌耐药性、生物利用度低和爆发释放等各种缺点。值得注意的是，局部使用具有持续释放特性的非抗生素药物可以有效地克服这些问题。有文献记载，绿原酸（CGA）具有良好的抗炎和抗氧化特性。为了实现 CGA 的持续释放，我们通过将 CGA 装载到聚（D，L-乳酸-乙醇酸）（PLGA）上并使用聚乙烯吡咯烷酮（PVP）对其进行修饰来合成 CGA-PLGA@PVP 纳米胶束，以获得更好的分散性。结果表明，CGA-PLGA@PVP 纳米胶束可以延长 CGA 的释放时间，不仅可以有效清除活性氧（ROS），还可以下调脂多糖（LPS）处理的 RAW264.7 细胞中促炎细胞因子的过度表达。此外，CGA-PLGA@PVP 纳米胶束在局部注射后可以在牙龈组织中保持 24 小时以上，抑制牙槽骨吸收并防止牙周炎在小鼠模型中的进展，表现出良好的生物相容性。因此，CGA-PLGA@PVP 纳米胶束具有很大的应用潜力，有望成为治疗牙周炎的一种新策略。
