Baker G B, Wong J T, Coutts R T, Pasutto F M
J Chromatogr. 1987 Apr 17;392:317-31. doi: 10.1016/s0021-9673(01)94276-1.
A method is described for simultaneous extraction and quantitation of the amines 2-phenylethylamine, tele-methylhistamine, histamine, tryptamine, m- and p-tyramine, 3-methoxytyramine, 5-hydroxytryptamine, cadaverine, putrescine, spermidine and spermine. This method is based on extractive derivatization of the amines with a perfluoroacylating agent, pentafluorobenzoyl chloride, under basic aqueous conditions. Analysis was done on a gas chromatograph equipped with an electron-capture detector and a capillary column system. The procedure is relatively rapid and provides derivatives with good chromatographic properties. Its application to analysis of the above amines in cheese and chocolate products is described.
本文描述了一种同时提取和定量分析2-苯乙胺、间甲基组胺、组胺、色胺、间酪氨酸和对酪氨酸、3-甲氧基酪胺、5-羟色胺、尸胺、腐胺、亚精胺和精胺的方法。该方法基于在碱性水溶液条件下，用全氟酰化剂五氟苯甲酰氯对胺类进行萃取衍生化。分析在配备电子捕获检测器和毛细管柱系统的气相色谱仪上进行。该方法相对快速，并能提供具有良好色谱性能的衍生物。文中还描述了该方法在奶酪和巧克力产品中上述胺类分析的应用。
