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Med Oral Patol Oral Cir Bucal. 2022 Sep 1;27(5):e468-e475. doi: 10.4317/medoral.25462.
We aimed to histomorphometrically evaluate the effects of Leucocyte-Platelet-Rich Fibrin (L-PRF), with and without the combination of a bone grafting material, for alveolar ridge preservation using an in vivo canine model.
Seven dogs (Female Beagles, ~18-month-old) were acquired for the study. L-PRF was prepared from each individual animal by drawing venous blood and spinning them through a centrifuge at 408 RCF-clot (IntrasSpin, Intra-Lock, Boca Raton, FL). L-PRF membranes were obtained from XPression fabrication kit (Biohorizons Implant Systems, Inc., AL, USA). A split mouth approach was adopted with the first molar mesial and distal socket defects treated in an interpolated fashion of the following study groups: 1) Empty socket (negative control); 2) OSS filled defect 3) L-PRF membrane; and 4) Mix of Bio-Oss® with L-PRF. After six weeks, samples were harvested, histologically processed, and evaluated for bone area fraction occupancy (BAFO), vertical/horizontal ridge dimensions (VRD and HRD, respectively), and area of coronal soft tissue infiltration.
BAFO was statistically lower for the control group in comparison to all treatment groups. Defects treated with Bio-Oss® were not statistically different then defects treated solely with L-PRF. Collapsed across all groups, L-PRF exhibited higher degrees of BAFO than groups without L-PRF. Defects filled with Bio-Oss® and Bio-Oss® with L-PRF demonstrated greater maintenance of VRD relative to the control group. Collapsed across all groups, Bio-Oss® maintained the VRD and resulted in less area of coronal soft tissue infiltration compared to the empty defect. Soft tissue infiltration observed at the coronal area was not statistically different among defects filled with L-PRF, Bio-Oss®, and Bio-Oss® with L-PRF.
Inclusion of L-PRF to particulate xenograft did not promote additional bone heading at 6 weeks in vivo. However, we noted that L-PRF alone promoted alveolar socket regeneration to levels comparable to particulate xenografts, suggesting its potential utilization for socket preservation.
本研究旨在通过体内犬模型，采用组织形态计量学方法评估富白细胞纤维蛋白（L-PRF）单独使用或与骨移植材料联合使用对牙槽嵴保存的效果。
本研究共纳入 7 只（雌性比格犬，约 18 月龄）。通过从每只动物抽取静脉血并在 408 RCF 条件下离心旋转（IntrasSpin，Intra-Lock，佛罗里达州博卡拉顿）来制备 L-PRF。L-PRF 膜从 XPression 制造试剂盒（Biohorizons Implant Systems，Inc.，AL，美国）获得。采用双侧上颌第一磨牙近中和远中牙槽窝缺损的方法，采用以下研究组的交错方式进行处理：1）空牙槽窝（阴性对照组）；2）OSS 填充缺损；3）L-PRF 膜；和 4）Bio-Oss®与 L-PRF 的混合物。6 周后，采集样本，进行组织学处理，并评估骨面积分数占有率（BAFO）、垂直/水平牙槽嵴尺寸（VRD 和 HRD，分别）以及冠部软组织浸润面积。
与所有治疗组相比，对照组的 BAFO 统计学上较低。与单独使用 L-PRF 的缺损相比，使用 Bio-Oss®治疗的缺损无统计学差异。所有组中，L-PRF 表现出比不含有 L-PRF 的组更高的 BAFO 程度。用 Bio-Oss®和 Bio-Oss®与 L-PRF 填充的缺损显示出与对照组相比，VRD 维持更好。所有组中，Bio-Oss®维持 VRD，并导致与空牙槽窝相比，冠部软组织浸润面积更小。在填充 L-PRF、Bio-Oss®和 Bio-Oss®与 L-PRF 的缺损中，观察到的冠部软组织浸润无统计学差异。
在体内 6 周时，将 L-PRF 加入颗粒状异种移植物中不会促进额外的骨生成。然而，我们注意到，单独使用 L-PRF 可促进牙槽窝再生至与颗粒状异种移植物相当的水平，这表明其在牙槽窝保存中的潜在应用。
