Department of Aesthetic Surgery, the Daqing Oilfield General Hospital, Daqing, Heilongjiang, China.
Department of Burns and Plastic Surgery, The Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing, Jiangsu, China.
Adipocyte. 2022 Dec;11(1):501-509. doi: 10.1080/21623945.2022.2107195.
In cell-assisted lipotransfer, adipose-derived stem cells play a crucial role in enhancing fat graft retention. , human adipose-derived stem cells were modified with Bcl-2 gene. In vivo, aspirated fat was mixed with the Bcl-2-modified adipose-derived stem cells and then transplanted subcutaneously into nude mice. The retention of fat graft was evaluated. The surviving Bcl-2-modified adipose-derived stem cells were tracked after transplantation. Capillary density was quantified after transplantation. Transplantation with Bcl-2-modified adipose-derived stem cells enhanced fat graft retention by 49% and 114% at 6 weeks compared with the Fat + vector-modified adipose-derived stem cell group and Fat-only group, respectively. Transplants from the Fat + Bcl-2-modified adipose-derived stem cell group had significantly more intact adipocytes and lower levels of fat necrosis and fibrosis at 6 weeks. The survival of Bcl-2-modified adipose-derived stem cells increased by 33% at 3 weeks and 54% at 6 weeks, respectively, compared with vector-modified adipose-derived stem cells. The capillary density was 24% higher in Fat + Bcl-2-modified adipose-derived stem cell group than in Fat + vector-modified adipose-derived stem cell group or 60% higher than in Fat-only group at 3 weeks.
在细胞辅助脂肪转移中，脂肪来源干细胞在增强脂肪移植物保留方面起着至关重要的作用。在这项研究中，用人的脂肪来源干细胞进行了 Bcl-2 基因修饰。在体内，将抽吸的脂肪与 Bcl-2 修饰的脂肪来源干细胞混合，然后皮下移植到裸鼠中。评估脂肪移植物的保留情况。移植后追踪存活的 Bcl-2 修饰的脂肪来源干细胞。移植后定量毛细血管密度。与脂肪+载体修饰的脂肪来源干细胞组和仅脂肪组相比，Bcl-2 修饰的脂肪来源干细胞移植分别使脂肪移植物保留增加了 49%和 114%，在第 6 周时。脂肪+ Bcl-2 修饰的脂肪来源干细胞组的移植体具有更多完整的脂肪细胞，并且在第 6 周时脂肪坏死和纤维化的水平较低。与载体修饰的脂肪来源干细胞相比，Bcl-2 修饰的脂肪来源干细胞的存活率在第 3 周时分别增加了 33%，在第 6 周时增加了 54%。在第 3 周时，脂肪+ Bcl-2 修饰的脂肪来源干细胞组的毛细血管密度比脂肪+载体修饰的脂肪来源干细胞组高 24%，比仅脂肪组高 60%。
