Stanford, Calif. From the Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, and the Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine.
Plast Reconstr Surg. 2015 Jul;136(1):67-75. doi: 10.1097/PRS.0000000000001367.
Cell-assisted lipotransfer has shown much promise as a technique for improving fat graft take. However, the concentration of stromal vascular fraction cells required to optimally enhance fat graft retention remains unknown.
Human lipoaspirate was processed for both fat transfer and harvest of stromal vascular fraction cells. Cells were then mixed back with fat at varying concentrations ranging from 10,000 to 10 million cells per 200 μl of fat. Fat graft volume retention was assessed by means of computed tomographic scanning over 8 weeks, and then fat grafts were explanted and compared histologically for overall architecture and vascularity.
Maximum fat graft retention was seen at a concentration of 10,000 cells per 200 μl of fat. The addition of higher number of cells negatively impacted fat graft retention, with supplementation of 10 million cells producing the lowest final volumes, lower than fat alone. Interestingly, fat grafts supplemented with 10,000 cells showed significantly increased vascularity and decreased inflammation, whereas fat grafts supplemented with 10 million cells showed significant lipodegeneration compared with fat alone
: The authors' study demonstrates dose dependence in the number of stromal vascular fraction cells that can be added to a fat graft to enhance retention. Although cell-assisted lipotransfer may help promote graft survival, this effect may need to be balanced with the increased metabolic load of added cells that may compete with adipocytes for nutrients during the postgraft period.
细胞辅助脂肪转移技术作为一种提高脂肪移植物成活率的技术，具有很大的潜力。然而，为了最佳地提高脂肪移植物的保留率，所需的基质血管成分细胞的浓度尚不清楚。
对人脂肪抽吸物进行脂肪转移和基质血管成分细胞收获。然后将细胞以每 200μl 脂肪 10000 到 1000 万细胞的不同浓度与脂肪混合。通过 8 周的计算机断层扫描评估脂肪移植物体积保留率，然后取出脂肪移植物并进行组织学比较，以评估整体结构和血管生成情况。
在每 200μl 脂肪中添加 10000 个细胞的浓度下，可获得最大的脂肪移植物保留率。添加更多数量的细胞会对脂肪移植物的保留产生负面影响，补充 1000 万个细胞会导致最终体积最低，低于单独使用脂肪的情况。有趣的是，与单独使用脂肪相比，添加 10000 个细胞的脂肪移植物表现出明显增加的血管生成和减少的炎症，而添加 1000 万个细胞的脂肪移植物则表现出明显的脂肪变性。
作者的研究表明，在添加到脂肪移植物中以提高保留率的基质血管成分细胞数量上存在剂量依赖性。尽管细胞辅助脂肪转移可能有助于促进移植物存活，但这种效果可能需要与添加细胞的代谢负荷相平衡，因为在移植物后期，这些细胞可能会与脂肪细胞争夺营养物质。
