Department of Obstetrics and Gynecology, Meir Medical Center, Kfar Saba, Israel.
Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Am J Reprod Immunol. 2022 Oct;88(4):e13598. doi: 10.1111/aji.13598. Epub 2022 Aug 17.
Preeclampsia (PE) and intrauterine growth restriction (IUGR) are leading causes of perinatal complications, affecting 8%-10% of all pregnancies. Inflammasomes are suspected to be one of the mechanisms that lead to the process of term and preterm labors. This study evaluated the inflammasome-dependent inflammation processes in placental tissue of women with PE and IUGR.
In this prospective cohort study, 14 women with PE, 15 with placental-related IUGR and 19 with normal pregnancy (NP) were recruited during admission for delivery. Maternal blood was obtained prior to delivery and neonatal cord blood and placental tissue were obtained after delivery.
NLRP7 and PYCARD protein expression were higher in placental PE and IUGR samples versus NP samples. Immunostaining revealed that NLRP7 and PYCARD were upregulated in PE and IUGR placental syncytiotrophoblast, stroma and endothelial cells. PYCARD serum levels were significantly higher in women with PE and IUGR. No significant changes were observed in neonatal cord blood.
NLRP7 and PYCARD are key inflammatory proteins that are significantly elevated in PE and IUGR. Better understanding their significance may enable them to become markers of prediction or progression of PE and IUGR.
子痫前期 (PE) 和宫内生长受限 (IUGR) 是围产期并发症的主要原因，影响所有妊娠的 8%-10%。炎症小体被怀疑是导致足月和早产分娩过程的机制之一。本研究评估了炎症小体依赖性炎症过程在患有 PE 和 IUGR 的妇女的胎盘组织中。
在这项前瞻性队列研究中，在分娩入院期间招募了 14 名患有 PE 的妇女、15 名与胎盘相关的 IUGR 妇女和 19 名正常妊娠 (NP) 妇女。在分娩前采集母体血液，在分娩后采集新生儿脐带血和胎盘组织。
与 NP 样本相比，PE 和 IUGR 胎盘样本中的 NLRP7 和 PYCARD 蛋白表达更高。免疫染色显示 NLRP7 和 PYCARD 在 PE 和 IUGR 胎盘合体滋养层、基质和内皮细胞中上调。PE 和 IUGR 妇女的 PYCARD 血清水平显著升高。新生儿脐带血中未观察到明显变化。
NLRP7 和 PYCARD 是关键的炎症蛋白，在 PE 和 IUGR 中显著升高。更好地了解它们的意义可能使它们成为预测或进展的标志物PE 和 IUGR。
