Department of Bioengineering, Faculty of Engineering, Bilecik Şeyh Edebali University, Bilecik, Turkey.
Department of Occupational Health and Safety, Faculty of Health Sciences, Bingöl University, Bingöl, Turkey.
Braz J Cardiovasc Surg. 2022 Aug 16;37(4):481-487. doi: 10.21470/1678-9741-2020-0378.
Presenilin 1 (PSEN1), catalase (CAT), glutathione-S-transferase (GST) and paraoxonase 1 (PON1) play a vital role in prediction, diagnosis and therapy of metabolic disorders.
Metabolic enzyme activities and lipid peroxidation in serum of cerebrovascular diseases (CVD) and coronary artery diseases were measured by spectrophotometric methods. mRNA was isolated from leukocytes of the patient group and healthy adult patients. Quantitative gene expression of PSEN1, CAT and GST mRNA was identified by quantitative real-time polymerase chain reaction (qPCR).
The PSEN1, CAT and GST expression in patients showed significant differences compared to the control group. PSEN1 expression in leukocytes was significantly about twice as high as that of the control group in patients with CVD. The GST, CAT and PON1 activity showed significant differences in patient groups compared to the control group.
The mRNA expression levels can be used as a potential biomarker in the diagnosis of atherosclerosis that occurs as a result of the metabolic disorder. In atherosclerotic patients, antioxidant status is independently related to an increased risk of cardiovascular events. Antioxidant activities and mRNA expressions may have predictive value, as well as available risk factors.
早老素 1（PSEN1）、过氧化氢酶（CAT）、谷胱甘肽-S-转移酶（GST）和对氧磷酶 1（PON1）在代谢紊乱的预测、诊断和治疗中起着至关重要的作用。
采用分光光度法测定脑血管病（CVD）和冠状动脉疾病患者血清中的代谢酶活性和脂质过氧化。从患者和健康成年患者的白细胞中分离 mRNA。采用实时定量聚合酶链反应（qPCR）鉴定 PSEN1、CAT 和 GST mRNA 的定量基因表达。
与对照组相比，患者的 PSEN1、CAT 和 GST 表达存在显著差异。CVD 患者白细胞中的 PSEN1 表达明显比对照组高约两倍。与对照组相比，患者组的 GST、CAT 和 PON1 活性存在显著差异。
mRNA 表达水平可用作代谢紊乱引起的动脉粥样硬化的潜在诊断生物标志物。在动脉粥样硬化患者中，抗氧化状态与心血管事件风险增加独立相关。抗氧化活性和 mRNA 表达可能具有预测价值，以及可用的风险因素。
