Tsuruta Y, Tomida H, Kohashi K, Ohkura Y
J Chromatogr. 1987 Apr 24;416(1):63-9. doi: 10.1016/0378-4347(87)80485-1.
A sensitive method for the simultaneous determination of urinary imidazoleacetic acid and N tau- and N pi-methylimidazoleacetic acids which employs high-performance liquid chromatography with fluorescence detection is described. The compounds were converted into the corresponding fluorescent esters by reaction with 4-bromomethyl-7-methoxycoumarin. These derivatives are separated by liquid chromatography on a Radial-Pak silica column. The detection limits for imidazoleacetic acid and N tau-and N pi-methylimidazoleacetic acids in urine are 15, 10 and 20 pmol/ml, respectively. The 24-h urinary excretion of imidazoleacetic acid and N tau-and N N pi-methylimidazoleacetic acids by healthy persons was 5.7-39.9, 4.3-24.6 and 1.5-19.3 nmol/mg of creatinine, respectively.
描述了一种采用高效液相色谱-荧光检测法同时测定尿中咪唑乙酸、Nτ-甲基咪唑乙酸和Nπ-甲基咪唑乙酸的灵敏方法。这些化合物通过与4-溴甲基-7-甲氧基香豆素反应转化为相应的荧光酯。这些衍生物在径向Pak硅胶柱上通过液相色谱分离。尿中咪唑乙酸、Nτ-甲基咪唑乙酸和Nπ-甲基咪唑乙酸的检测限分别为15、10和20 pmol/ml。健康人24小时尿中咪唑乙酸、Nτ-甲基咪唑乙酸和Nπ-甲基咪唑乙酸的排泄量分别为5.7 - 39.9、4.3 - 24.6和1.5 - 19.3 nmol/mg肌酐。
