Keyzer J J, Wolthers B G, Breukelman H, Kauffman H F, de Monchy J G
Clin Chim Acta. 1982 Jun 3;121(3):379-87. doi: 10.1016/0009-8981(82)90247-9.
N tau-Methylimidazoleacetic acid, the quantitatively most important metabolite of histamine, was isolated from urine by ion exchange chromatography. After esterification with 2-propanol and extraction, N tao-methylimidazoleacetic acid was analyzed by capillary gas chromatography with nitrogen-phosphorus detection, using N tao-ethylimidazoleacetic acid as internal standard. The synthesis of this internal standard is described. In contrast to the methods hitherto described, this method is appropriate for use in clinical chemical laboratories. Normal 24-h excretion ranged from 8.3 to 18.5 mumol (n = 20). Five patients with mastocytosis, a patient with chronic myelocytic leukemia and a patient after an anaphylactoid reaction on acetylsalicylic acid showed highly elevated values.
Nτ-甲基咪唑乙酸是组胺在定量方面最重要的代谢产物，通过离子交换色谱法从尿液中分离出来。用2-丙醇酯化并萃取后，以Nτ-乙基咪唑乙酸为内标，采用氮磷检测的毛细管气相色谱法对Nτ-甲基咪唑乙酸进行分析。文中描述了该内标的合成方法。与迄今所描述的方法不同，该方法适用于临床化学实验室。正常24小时排泄量范围为8.3至18.5微摩尔（n = 20）。五名肥大细胞增多症患者、一名慢性粒细胞白血病患者以及一名对乙酰水杨酸发生类过敏反应后的患者，其排泄量值显著升高。
