School of Physical Science and Technology, Ningbo University, Ningbo 315211, Zhejiang, China.
The Interdisciplinary Research Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
Nano Lett. 2022 Sep 14;22(17):7173-7179. doi: 10.1021/acs.nanolett.2c02447. Epub 2022 Aug 17.
A myriad of DNA origami nanostructures have been demonstrated in various intriguing applications. In pursuit of facile yet high-yield synthesis, the mechanisms underlying DNA origami folding need to be resolved. Here, we visualize the folding processes of several multidomain DNA origami structures under ambient annealing conditions in solution using atomic force microscopy with submolecular resolution. We reveal the coexistence of diverse transitional structures that might result in the same prescribed products. Based on the experimental observations and the simulation of the energy landscapes, we propose the heterogeneity of the folding pathways of multidomain DNA origami structures. Our findings may contribute to understanding the high-yield folding mechanism of DNA origami.
已经有许多 DNA 折纸纳米结构在各种有趣的应用中得到了展示。为了实现简单而高产的合成，需要解决 DNA 折纸折叠的机制。在这里，我们使用原子力显微镜在亚分子分辨率下，在溶液中观察到几种多域 DNA 折纸结构在环境退火条件下的折叠过程。我们揭示了可能导致相同规定产物的不同过渡结构的共存。基于实验观察和能量景观的模拟，我们提出了多域 DNA 折纸结构折叠途径的异质性。我们的发现可能有助于理解 DNA 折纸的高产折叠机制。
