Physics Department, Technische Universität München, Am Coulombwall 4a, Garching, Germany.
Adv Exp Med Biol. 2012;733:87-96. doi: 10.1007/978-94-007-2555-3_9.
DNA origami is a revolutionary method for the assembly of molecular nanostructures from DNA with precisely defined dimensions and with an unprecedented yield. This can be utilized to arrange nanoscale components such as proteins or nanoparticles into pre-defined patterns. For applications it will now be of interest to arrange such components into functional complexes and study their geometry-dependent interactions. While commonly DNA nanostructures are characterized by atomic force microscopy or electron microscopy, these techniques often lack the time-resolution to study dynamic processes. It is therefore of considerable interest to also apply fluorescence microscopic techniques to DNA nanostructures. Of particular importance here is the utilization of novel super-resolved microscopy methods that enable imaging beyond the classical diffraction limit.
DNA 折纸术是一种从 DNA 组装分子纳米结构的革命性方法，具有精确定义的尺寸和前所未有的产率。这可以用来将纳米级组件（如蛋白质或纳米粒子）排列成预定的图案。对于应用，现在将感兴趣的是将这些组件排列成功能复合物，并研究它们的几何相关相互作用。虽然通常 DNA 纳米结构通过原子力显微镜或电子显微镜来表征，但这些技术通常缺乏研究动态过程的时间分辨率。因此，将荧光显微镜技术应用于 DNA 纳米结构也具有相当大的意义。这里特别重要的是利用能够超越经典衍射极限的新型超分辨率显微镜方法。
