Naghdi Elahe, Moran Griffin E, Reinau Marika Ejby, De Malsche Wim, Neusüß Christian
Department of Chemistry, Aalen University, Aalen, Germany.
Novo Nordisk A/S, Global Research Technologies, Maaloev, Denmark.
Electrophoresis. 2023 Jan;44(1-2):246-267. doi: 10.1002/elps.202200179. Epub 2022 Sep 4.
The online coupling of microchip electrophoresis (ME) as a fast, highly efficient, and low-cost miniaturized separation technique to mass spectrometry (MS) as an information-rich and sensitive characterization technique results in ME-MS an attractive tool for various applications. In this paper, we review the basic concepts and latest advances in technology for ME coupled to MS during the period of 2016-2021, covering microchip materials, structures, fabrication techniques, and interfacing to electrospray ionization (ESI)-MS and matrix-assisted laser desorption/ionization-MS. Two critical issues in coupling ME and ESI-MS include the electrical connection used to define the electrophoretic field strength along the separation channel and the generation of the electrospray for MS detection, as well as, a miniaturized ESI-tip. The recent commercialization of ME-MS in zone electrophoresis and isoelectric focusing modes has led to the widespread application of these techniques in academia and industry. Here we summarize recent applications of ME-MS for the separation and detection of antibodies, proteins, peptides, carbohydrates, metabolites, and so on. Throughout the paper these applications are discussed in the context of benefits and limitations of ME-MS in comparison to alternative techniques.
将微芯片电泳（ME）这种快速、高效且低成本的微型分离技术与作为信息丰富且灵敏表征技术的质谱（MS）进行在线联用，使得ME-MS成为一种适用于各种应用的有吸引力的工具。在本文中，我们综述了2016年至2021年期间ME与MS联用的基本概念和最新技术进展，涵盖微芯片材料、结构、制造技术以及与电喷雾电离（ESI）-MS和基质辅助激光解吸/电离-MS的接口。ME与ESI-MS联用中的两个关键问题包括用于定义沿分离通道的电场强度的电连接以及用于MS检测的电喷雾的产生，还有一个微型化的ESI尖端。ME-MS在区带电泳和等电聚焦模式下的近期商业化已导致这些技术在学术界和工业界的广泛应用。在此我们总结了ME-MS在抗体、蛋白质、肽、碳水化合物、代谢物等的分离和检测方面的近期应用。在整篇论文中，这些应用是在将ME-MS与替代技术相比较的优势和局限性的背景下进行讨论的。
