Sun S R
J Pharm Sci. 1978 Oct;67(10):1413-5. doi: 10.1002/jps.2600671022.
A sensitive fluorescence--TLC densitometric procedure was developed for the specific determination of diazepam (I) and its two metabolites, desmethyldiazepam (II) and oxazepam (III), in serum. After extraction from serum with benzene, the compounds were separated by TLC and converted with a sulfuric acid spray to greenish-yellow fluorescence spots with Rf values of 0.72, 0.43, and 0.17, respectively. Quantitation of the TLC plate was accomplished by scanning with a densitometer at 380 nm. The sensitivities of the assay were 18 (I), 6 (II), and 5 (III) ng/ml of serum. This procedure was also was applicable to other 1,4-benzodiazepines in biological fluids.
建立了一种灵敏的荧光 - 薄层色谱光密度法，用于特异性测定血清中地西泮（I）及其两种代谢物去甲基西泮（II）和奥沙西泮（III）。用苯从血清中提取后，通过薄层色谱分离这些化合物，并用硫酸喷雾转化为黄绿色荧光斑点，其比移值分别为0.72、0.43和0.17。通过用密度计在380nm处扫描完成薄层色谱板的定量。该测定法的灵敏度分别为血清中18（I）、6（II）和5（III）ng/ml。该方法也适用于生物体液中的其他1,4 - 苯二氮䓬类药物。
