Sun S R
J Pharm Sci. 1978 May;67(5):639-41. doi: 10.1002/jps.2600670517.
A sensitive fluorescence TLC--densitometric procedure was developed for the specific determination of chlordiazepoxide (I) and its two metabolites, demoxepam (II) and desmethylchlordiazepoxide (III), in serum. After extraction from serum with ether, I, II, and II were separated by TLC and converted with a sulfuric acid spray to pale blue (Rf 0.63), green (Rf 0.54), and blue (Rf 0.45) fluorescence spots, respectively. Quantitation was accomplished by scanning the plate with a densitometer at 390 (I), 430 (II), and 390 (III) nm. The sensitivities were 0.05 (I), 0.01 (II), and 0.01 (III) microgram/ml of serum. The procedure was successfully applied to measurement of I-III in human serum after oral administration of 20 mg of chlordiazepoxide hydrochloride.
已开发出一种灵敏的荧光薄层色谱 - 光密度测定法，用于特异性测定血清中的氯氮䓬（I）及其两种代谢物去甲地西泮（II）和去甲基氯氮䓬（III）。用乙醚从血清中提取后，I、II 和 III 通过薄层色谱分离，并用硫酸喷雾转化为浅蓝色（比移值0.63）、绿色（比移值0.54）和蓝色（比移值0.45）荧光斑点。通过用密度计在390nm（I）、430nm（II）和390nm（III）处扫描板来进行定量。灵敏度分别为0.05（I）、0.01（II）和0.01（III）微克/毫升血清。该方法成功应用于口服20mg盐酸氯氮䓬后人体血清中I - III的测定。
