Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong.
City University of Hong Kong Shenzhen Research Institute, Shenzhen, China.
J Antimicrob Chemother. 2022 Oct 28;77(11):3039-3049. doi: 10.1093/jac/dkac276.
Carbapenemase-producing Vibrio spp., which exhibit an XDR phenotype, have become increasingly prevalent and pose a severe threat to public health.
To investigate the genetic characteristics of NDM-1-producing Vibrio spp. isolates and the dissemination mechanisms of blaNDM-1 in Vibrio.
A total of 1363 non-duplicate Vibrio spp. isolates collected from shrimp samples in China were subjected to antimicrobial susceptibility tests and screened for blaNDM-1. The blaNDM-1-positive isolates were further characterized by PFGE, MLST, conjugation and WGS using Illumina and Nanopore platforms. Plasmid stability and fitness cost were assessed using Escherichia coli J53, Klebsiella pneumoniae Kpt80 and Salmonella spp. SA2051 as recipient strains.
In total, 13 blaNDM-1-positive isolates were identified, all exhibiting MDR. WGS analysis revealed that the 13 blaNDM-1 genes were all associated with a derivative of Tn125. Plasmid analysis revealed that six blaNDM-1 genes were located in IncC plasmids and the other seven were carried by plasmids of two different novel types. Conjugation and plasmid stability assays showed that only the IncC plasmids could be transferred to all the recipient strains and could be stably maintained in the hosts.
The emergence of the novel plasmids has contributed to the variable genetic contexts of blaNDM-1 in Vibrio spp. and IncC plasmids harbouring the blaNDM-1 gene could facilitate the spread of such genes between Vibrio spp. and other zoonotic pathogens, leading to a rapid dissemination of blaNDM-1 in bacterial pathogens worldwide.
具有广泛耐药性表型的产碳青霉烯酶弧菌越来越普遍，对公共卫生构成严重威胁。
研究产 NDM-1 弧菌分离株的遗传特征及 blaNDM-1 在弧菌中的传播机制。
对中国虾样中分离的 1363 株非重复弧菌进行药敏试验和 blaNDM-1 筛选。对 blaNDM-1 阳性分离株进行 PFGE、MLST、接合和 Illumina 和 Nanopore 平台的 WGS 分析。使用大肠杆菌 J53、肺炎克雷伯菌 Kpt80 和沙门氏菌 SA2051 作为受体菌株评估质粒稳定性和适合度代价。
共鉴定出 13 株 blaNDM-1 阳性分离株，均表现为 MDR。WGS 分析显示，13 株 blaNDM-1 基因均与 Tn125 的衍生物有关。质粒分析显示，6 株 blaNDM-1 基因位于 IncC 质粒上，其余 7 株位于两种不同新型质粒上。接合和质粒稳定性试验表明，只有 IncC 质粒可以转移到所有受体菌株，并能在宿主中稳定维持。
新型质粒的出现导致 blaNDM-1 在弧菌中的遗传背景多样化。携带 blaNDM-1 基因的 IncC 质粒可促进该基因在弧菌和其他人畜共患病原体之间的传播，导致 blaNDM-1 在全球细菌病原体中的快速传播。
