Chen Dan, Sun Ruanyang, Wang Jing, Chen Kaichao, Xie Miaomiao, Lin Qinlu, Li Jinwang, Chen Sheng, Liu Xiaobo
National Engineering Laboratory for Deep Process of Rice and By-Products, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, China.
Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, China.
Appl Microbiol Biotechnol. 2025 Aug 21;109(1):189. doi: 10.1007/s00253-025-13567-2.
Carbapenem-resistant Vibrio parahaemolyticus has emerged and spread extensively in China, posing a substantial threat to food safety and human health. This study investigated the prevalence of carbapenem resistance in V. parahaemolyticus strains recovered from various types of food samples in China, and the underlying mechanisms of transmission of the bla gene harbored by such strains. In this work, a total of 230 (62.1%) strains were isolated from seafood samples collected in Changsha, Hunan Province; among them, 47 strains were found to be cefotaxime-resistant. These strains were subjected to whole-genome sequencing and found to exhibit significant genetic diversity; among them, 13 strains were found to carry the bla gene. Nanopore sequencing showed that the bla gene was located in the chromosome in strain VP288 but was plasmid-borne in the other 12 strains. All bla-bearing plasmids were of the IncC type that harbored a conserved backbone. However, the multidrug resistance regions differed significantly from each other and could be classified into four groups: (1) the plasmid pVP181-NDM was the longest and carried an additional Tn7 transposase gene; (2) both pVP156-NDM and pVP209-NDM contained two structurally different resistance regions; (3) both pVP205-NDM and pVP148-NDM harbored two bla genes flanked by three copies of ISCR1 insertion elements; (4) the plasmids pVP228-NDM and pVP14-NDM contained an MDR region similar to that of pVP181-NDM. The high-level carbapenem resistance phenotype exhibited by foodborne V. parahaemolyticus underscores an urgent need for the development of effective control strategies and surveillance to limit the spread of the carbapenem resistance determinants carried by these strains. KEY POINTS: • The rate of resistance of food-borne V. parahaemolyticus strains to carbapenems was at a concerning level. • The bla gene was located in IncC-type plasmids which shared a conserved backbone. • The MDR profiles of bla-bearing plasmids varied significantly.
耐碳青霉烯类副溶血性弧菌已在中国出现并广泛传播，对食品安全和人类健康构成重大威胁。本研究调查了从中国各类食品样本中分离出的副溶血性弧菌菌株对碳青霉烯类抗生素的耐药率，以及此类菌株携带的bla基因的潜在传播机制。在这项工作中，共从湖南省长沙市采集的海鲜样本中分离出230株（62.1%）菌株；其中，47株对头孢噻肟耐药。对这些菌株进行全基因组测序，发现它们具有显著的遗传多样性；其中，13株携带bla基因。纳米孔测序显示，bla基因在菌株VP288中位于染色体上，但在其他12株中是质粒携带的。所有携带bla的质粒均为IncC型，具有保守的骨架。然而，多药耐药区域彼此差异显著，可分为四组：（1）质粒pVP181-NDM最长，还携带一个额外的Tn7转座酶基因；（2）pVP156-NDM和pVP209-NDM均包含两个结构不同的耐药区域；（3）pVP205-NDM和pVP148-NDM均携带两个bla基因，两侧有三个拷贝的ISCR1插入元件；（4）质粒pVP228-NDM和pVP14-NDM包含一个与pVP181-NDM相似的多药耐药区域。食源性副溶血性弧菌表现出的高水平碳青霉烯类抗生素耐药表型凸显了迫切需要制定有效的控制策略和监测措施，以限制这些菌株携带的碳青霉烯类抗生素耐药决定因素的传播。要点：•食源性副溶血性弧菌菌株对碳青霉烯类抗生素的耐药率处于令人担忧的水平。•bla基因位于具有保守骨架的IncC型质粒中。•携带bla的质粒的多药耐药谱差异显著。
