Concentration and estimated flow of peptides from the rumen of dairy cattle: effects of protein quantity, protein solubility, and feeding frequency.

Experiments were conducted to ascertain the effect of dietary protein (type and amount) and feeding frequency on peptide concentration and flow from the rumen of lactating Holstein cows. When rations supplemented with soybean meal were fed 12 times/d and CP was 14.5, 17.1, or 20.6%, the estimated flow of N from the rumen was 22, 33, and 34 g/d, respectively. When the soybean meal was autoclaved to decrease protein solubility (14.4, 16.9, and 19.3% CP the flow of peptide N was 19, 26, and 31 g/d, respectively. In both of these experiments rumen volume and dilution rate remained relatively constant throughout the day, and flow was proportional to concentration. If the cows were fed rations containing 17.8% CP once a day, the peptide concentration and dilution rate were highest 2 h after feeding and declined significantly thereafter. Replacement of half the soybean protein with either extruded soybean meal or fish meal decreased the flow of peptide N from the rumen (19 versus 14 and 15 g/d, respectively). Because the total flow of soluble protein and ammonia as well as peptides was less if cows were fed once a day than 12 times per day, increased feeding frequency appeared to increase ruminal proteolysis.