基于大环分子的抗冰晶形成冷冻保护剂及其在细胞冷冻保存中的应用。
Macrocycle molecule-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation.
机构信息
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Hebei University), Ministry of Education, Key Laboratory of Chemical Biology of Hebei Province, Hebei University, Baoding, 071002, P. R. China.
出版信息
J Mater Chem B. 2022 Sep 21;10(36):6922-6927. doi: 10.1039/d2tb01083f.
Cyclodextrin-based cryoprotectants were developed. α-TMCD, which can be easily put into large-scale production, showed enhanced cell viabilities of 19.97 ± 0.78%, 13.93 ± 4.46% and 19.10 ± 0.95% against GES-1, hucMSCs and A549 cells. Moreover, the viable cells observed by light microscope imaging showed that the enhanced hucMSC cell number percentage of α-TMCD was 103.2%. An α-TMCD-DMSO-based CPA exhibited an enhanced cryoprotective effect by a mechanism of DMSO-enhanced cell penetrating effect and α-TMCD-DMSO synergistically enhanced IMA ability. α-TMCD exhibited potential for the discovery of macrocycle-molecule-based cryoprotectants.
基于环糊精的冷冻保护剂被开发出来。α-TMCD 可以很容易地投入大规模生产，对 GES-1、hucMSCs 和 A549 细胞的细胞活力分别提高了 19.97±0.78%、13.93±4.46%和 19.10±0.95%。此外，通过光学显微镜成像观察到存活细胞，α-TMCD 增强的 hucMSC 细胞数量百分比为 103.2%。一种基于α-TMCD-DMSO 的复合冷冻保护剂通过 DMSO 增强细胞穿透作用的机制和 α-TMCD-DMSO 协同增强 IMA 能力表现出增强的冷冻保护效果。α-TMCD 为发现大环分子基冷冻保护剂提供了潜力。
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