基于杯芳烃的抗冰晶重结晶保护剂及其在细胞冷冻保存中的应用。

Calixarene-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation.

机构信息

College of Chemistry and Materials Science, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Ministry of Education), State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Chemical Biology of Hebei Province, Hebei University, Baoding, 071002, China.

College of Chemistry, Key Laboratory of Functional Polymer Materials (Ministry of Education), State Key Laboratory of Elemento-Organic Chemistry, Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin 300071, China.

出版信息

J Mater Chem B. 2023 Dec 6;11(47):11222-11227. doi: 10.1039/d3tb02432f.

DOI:10.1039/d3tb02432f

PMID:38013489

Abstract

The development of new cryoprotectants for cryopreservation of cells has attracted considerable interest. Herein, five calixarene-based CPAs (SC4A, S-S-C4A, S-SO-C4A, SBAC4A, and CAC4A) were developed, and their IRI activity, DIS property and cryoprotective effect were studied. SBAC4A with a sulphobetaine zwitterion and SC4A with sulfo group modification possessed better cryoprotective effects than the other calixarene-based CPAs, especially for SBAC4A with the enhanced cell viabilities of 16.16 ± 1.78%, 12.60 ± 1.15% and 14.90 ± 1.66% against MCF-7, hucMSCs and A549 cells, respectively. This result provides a supramolecular principle for developing novel CPAs with consideration of the factors of hydrogen bonding, the macromolecular crowding principle and the three-dimensional (3D) structure.

摘要

新型细胞低温保存保护剂的开发引起了广泛关注。本文开发了五种基于杯芳烃的 CPAs（SC4A、S-S-C4A、S-SO-C4A、SBAC4A 和 CAC4A），并研究了它们的 IRI 活性、DIS 性质和低温保护效果。带有磺基甜菜碱两性离子的 SBAC4A 和带有磺酸基团修饰的 SC4A 比其他基于杯芳烃的 CPAs 具有更好的低温保护效果，特别是对于 SBAC4A，对 MCF-7、hucMSCs 和 A549 细胞的细胞活力分别增强了 16.16±1.78%、12.60±1.15%和 14.90±1.66%。这一结果为开发新型 CPAs 提供了一个超分子原理，考虑了氢键、大分子拥挤原理和三维（3D）结构等因素。