Pan Jing, Zhang Yuan-Yuan, Jiao Xia-Xia, Song Lei-Na, Lin Cai-Qin, Wang Su-Li, Zhu Bin, Pan Shao-Ying, Ding Zhi-Yong, Zhao Wen-Li
Anhui University of Science and Technology of Medical College, Huainan 232000, Anhui Province, China.
Hubei University of Medicine, Shiyan 442000, Hubei Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2022 Aug;30(4):1071-1078. doi: 10.19746/j.cnki.issn.1009-2137.2022.04.015.
To investigate the expression of CD47 molecules in patients with newly diagnosis of adult acute myeloid leukemia (AML) and its correlation with clinical prognosis.
20 patients with acute myeloid leukemia diagnosed in Shanghai Fengxian District Central hospital from April 2020 to October 2021 and 5 cases with non malignant hematological diseases in the control group were collected, and the expression of CD47 in single nuclear cells of bone marrow and peripheral blood was detected by real-time fluorescence quantitative polymerase chain reaction (qPCR). Combined with the blood image, bone marrow smears, flow cytometry, chromosome and gene detection, ECOG score, etc. during the patient's initial diagnosis, the relationship between the patient's prognosis and CD47 was evaluated.
The expression of CD47 in bone marrow (P=0.0115) and peripheral blood mononuclear cells (P=0.0069) in new diagnosis AML patients was significantly higher than that of controls. In bone marrow mononuclear cells, the total survival time of patients with high CD47 expression was less than that of CD47 low expression patients (P=0.036). There was statistical significance in difference stratification group (P=0.012), but there was no statistical significance between CD47 expression and survival time in peripheral blood mononuclear cells (P=0.116). There were no statistical significance between bone marrow mononuclear cell CD47 expression and gene mutation fusion genes related to leukemia , CD34, CD38, CD123 (P>0.05). The proportion of bone marrow protocells in AML patients was >50%, the ECOG score was >2 points, MLLELL fusion gene and chromosome prognosis stratification were all risk factors affecting the survival of patients (P=0023, 0.036, 0.012, 0.001, respectively). The high expression of bone marrow CD47 in AML patients indicated a high risk of recurrence (P=0.017).
The high expression of bone marrow mononuclear cell CD47 in AML patients indicates poorer survival and higher risk of recurrence.
探讨新诊断的成人急性髓系白血病（AML）患者CD47分子的表达情况及其与临床预后的相关性。
收集2020年4月至2021年10月在上海市奉贤区中心医院确诊的急性髓系白血病患者20例及对照组非恶性血液病患者5例，采用实时荧光定量聚合酶链反应（qPCR）检测骨髓及外周血单个核细胞中CD47的表达。结合患者初诊时的血常规、骨髓涂片、流式细胞术、染色体及基因检测、ECOG评分等，评估患者预后与CD47的关系。
新诊断AML患者骨髓（P = 0.0115）及外周血单个核细胞（P = 0.0069）中CD47的表达明显高于对照组。在骨髓单个核细胞中，CD47高表达患者的总生存时间短于CD47低表达患者（P = 0.036）。差异分层组有统计学意义（P = 0.012），但外周血单个核细胞中CD47表达与生存时间无统计学意义（P = 0.116）。骨髓单个核细胞CD47表达与白血病相关基因突变融合基因、CD34、CD38、CD123之间无统计学意义（P>0.05）。AML患者骨髓原细胞比例>50%、ECOG评分>2分、MLLELL融合基因及染色体预后分层均为影响患者生存的危险因素（P分别为0023、0.036、0.012、0.001）。AML患者骨髓CD47高表达提示复发风险高（P = 0.017）。
AML患者骨髓单个核细胞CD47高表达提示生存较差且复发风险较高。
