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[10例ABO血型鉴定困难样本的基因测序分析]

[Gene Sequencing Analyses of 10 ABO Ambiguous Blood Group Samples].

作者信息

Huang Hui Ni, Mo Zhu Ning, Liao Xiang Cheng, Yang Zi Ji, Wei Yue Rong, Zhu Chun Li, Li Hai Lan

机构信息

Department of Blood Transfusion, Guangxi Zhuang Autonomous Region People's Hospital, Nanning 530021, Guangxi Zhuang Autonomous Region, China.

Department of Blood Transfusion, Guangxi Zhuang Autonomous Region People's Hospital, Nanning 530021, Guangxi Zhuang Autonomous Region, China,Email:feiyang2808 @163.com.

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2022 Aug;30(4):1193-1197. doi: 10.19746/j.cnki.issn.1009-2137.2022.04.034.

DOI:10.19746/j.cnki.issn.1009-2137.2022.04.034
PMID:35981383
Abstract

OBJECTIVE

Through analysis of ABO blood group gene typing technology, to assist in the identification of difficult clinical serological specimens.

METHODS

A total of 10 forwardreverse typing ambiguous samples were collected from January 2021 to August 2021 in our hospital.ABO genotypes were analysed by gene sequencing.

RESULTS

The genotypes of 10 ABO ambiguous blood group samples were A102/BW11, A102/BW12, O02/O02, A102/B303, A102/B101, BW11/O02, B101/O04, BW11/O01, BW11/O01, A101/O02, respectively. The genotype results of 6 cases was consistent with the serological phenotype, and the serological phenotype of 4 cases were different from the geno sequencing.

CONCLUSION

ABO blood groups genotyping technology combined with serological typing can be used for accurate typing of ambiguous blood group, and better ensure the safety of blood transfusion.

摘要

目的

通过分析ABO血型基因分型技术，辅助鉴定临床疑难血清学标本。

方法

收集我院2021年1月至2021年8月共10例正反定型不明确的样本，采用基因测序法分析ABO基因型。

结果

10例ABO血型不明确样本的基因型分别为A102/BW11、A102/BW12、O02/O02、A102/B303、A102/B101、BW11/O02、B101/O04、BW11/O01、BW11/O01、A101/O02。6例基因型结果与血清学表型一致，4例血清学表型与基因测序结果不同。

结论

ABO血型基因分型技术联合血清学分型可用于血型不明确样本的准确分型，更好地确保输血安全。

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