[Correlative Analysis between Production of Platelet HLA-Ⅰ Antibody and HLA-A, B Genes in Patients with Malignant Hematological Diseases].

OBJECTIVE

To investigate the correlation between the production of platelet HLA-Ⅰ antibody and HLA-A, B genes in patients with malignant hematological diseases, and explore the susceptible gene for producing platelet HLA-Ⅰ antibody.

METHODS

Patients with malignant hematological diseases who had received multiple platelet transfusion were selected as the research objects in the Department of Hematology of our hospital. Platelet HLA-I antibody were screened by ELISA, and the patients were divided into positive and negative groups according to the results. HLA-A and B genes were sequenced after genomic DNA was extracted, and the frequencies of them were compared between the two groups.

RESULTS

The positive rate of platelet HLA-I antibody was 22.95%. A total of 13 HLA-A alleles and 14 HLA-B alleles were obtained after the HLA-A and B genes sequencing in 100 cases. The frequencies of HLA-A24, HLA-A30, and HLA-B13 were significantly different between the two groups (P<0.05). Frequencies of HLA-A30 and HLA-B13 in the positive group were lower than those in the negative group (RR=0.107, 0.387), but HLA-A24 was higher (RR=1.412). After high-resolution typing of HLA-A24, HLA-A30, and HLA-B13, frequencies of HLA-A24∶02, HLA-A30∶01, and HLA-B13∶02 were significantly different between the two groups, the RR value was 1.412, 0.107, and 0.125, 95%CI was 0.961-2.075, 0.016-0.721, and 0.300-0.515, respectively.

CONCLUSION

HLA-A24∶02 may be a susceptible gene for producing platelet HLA-Ⅰ antibody in patients with malignant hematological diseases, while HLA-A30∶01 and HLA-B*13∶02 may be two protective genes.