Gao Xiao-Yun, Kou Li-Duo, Tian Hua, Wang Xin-Hua
Department of Blood Transfusion, Aerospace Center Hospital, Beijing 100049, China.
Department of Blood Transfusion, Aerospace Center Hospital, Beijing 100049, China.E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2022 Aug;30(4):1203-1207. doi: 10.19746/j.cnki.issn.1009-2137.2022.04.036.
To investigate the correlation between the production of platelet HLA-Ⅰ antibody and HLA-A, B genes in patients with malignant hematological diseases, and explore the susceptible gene for producing platelet HLA-Ⅰ antibody.
Patients with malignant hematological diseases who had received multiple platelet transfusion were selected as the research objects in the Department of Hematology of our hospital. Platelet HLA-I antibody were screened by ELISA, and the patients were divided into positive and negative groups according to the results. HLA-A and B genes were sequenced after genomic DNA was extracted, and the frequencies of them were compared between the two groups.
The positive rate of platelet HLA-I antibody was 22.95%. A total of 13 HLA-A alleles and 14 HLA-B alleles were obtained after the HLA-A and B genes sequencing in 100 cases. The frequencies of HLA-A24, HLA-A30, and HLA-B13 were significantly different between the two groups (P<0.05). Frequencies of HLA-A30 and HLA-B13 in the positive group were lower than those in the negative group (RR=0.107, 0.387), but HLA-A24 was higher (RR=1.412). After high-resolution typing of HLA-A24, HLA-A30, and HLA-B13, frequencies of HLA-A24∶02, HLA-A30∶01, and HLA-B13∶02 were significantly different between the two groups, the RR value was 1.412, 0.107, and 0.125, 95%CI was 0.961-2.075, 0.016-0.721, and 0.300-0.515, respectively.
HLA-A24∶02 may be a susceptible gene for producing platelet HLA-Ⅰ antibody in patients with malignant hematological diseases, while HLA-A30∶01 and HLA-B*13∶02 may be two protective genes.
探讨恶性血液病患者血小板HLA-Ⅰ类抗体产生与HLA-A、B基因的相关性，探寻产生血小板HLA-Ⅰ类抗体的易感基因。
选取我院血液科接受多次血小板输注的恶性血液病患者作为研究对象。采用ELISA法筛选血小板HLA-I抗体，根据结果将患者分为阳性组和阴性组。提取基因组DNA后对HLA-A和B基因进行测序，比较两组基因频率。
血小板HLA-I抗体阳性率为22.95%。100例患者HLA-A和B基因测序后共获得13个HLA-A等位基因和14个HLA-B等位基因。两组间HLA-A24、HLA-A30及HLA-B13基因频率差异有统计学意义（P<0.05）。阳性组HLA-A30和HLA-B13基因频率低于阴性组（RR=0.107，0.387），而HLA-A24高于阴性组（RR=1.412）。对HLA-A24、HLA-A30及HLA-B13进行高分辨分型后，两组间HLA-A24∶02、HLA-A30∶01及HLA-B13∶02基因频率差异有统计学意义，RR值分别为1.412、0.107和0.125，95%CI分别为0.961 - 2.075、0.016 - 0.721和0.300 - 0.515。
HLA-A24∶02可能是恶性血液病患者产生血小板HLA-Ⅰ类抗体的易感基因，而HLA-A30∶01和HLA-B*13∶02可能是两个保护性基因。
