Department of Biological Sciences, College of Sciences, Afe Babalola University, PMB 5454, Ado-Ekiti, Ekiti State, Nigeria.
Department of Food Science and Microbiology, College of Pure and Applied Sciences, Landmark University, PMB 1001, Omu-Aran, Kwara State, Nigeria.
Sci Rep. 2022 Aug 18;12(1):14106. doi: 10.1038/s41598-022-18555-4.
The germinability and protective potential of leaf infusion of Parkia biglobosa, Moringa oleifera and Vernonia amygdalina leaves on okra (Abelmoschus esculentus) seeds against infections simulated with suspended cells of Aspergillus niger, A. flavus, A. fumigatus, and Penicillium sp. were examined. Prior to planting, the okra seeds were first surface-sterilized in 5% sodium hypochlorite solution before steeping in known concentrations (0, 20, 40, 60, 80, and 100%) of the respective leaf infusions for a known duration. Seven of the steeped seeds were planted in plastic transparent containers, incubated for 7 days under light, and observed daily. Germination index, germination rate, germination time, and vigor index were calculated for each treatment, using standard procedures. The effective concentrations of the infusions of V. amygdalina, P. biglobosa and M. oleifera were 40, 40, and 60% respectively. Optimum steeping durations in leaf infusions were 1, 5, and 6 h, for P. biglobosa, M. oleifera and V. amygdalina, respectively. All the leaf infusions were observed to protect the okra seeds against infections with the test organisms. Furthermore, seeds steeped in the respective leaf infusions showed remarkably higher germinability potential than the control seeds steeped in water. The study confirmed that the leaf infusions may be attractive as economic alternatives for seed priming and protection.
研究了大托叶云实、辣木和鸭脚木叶浸提液对秋葵（Abelmoschus esculentus）种子的发芽能力和保护潜力，这些浸提液可模拟悬浮细胞接种的黑曲霉、黄曲霉、烟曲霉和青霉对种子的感染。在种植前，将秋葵种子先用 5%次氯酸钠溶液表面消毒，然后在已知浓度（0、20、40、60、80 和 100%）的各叶浸提液中浸泡已知时间。将 7 颗浸泡过的种子种植在塑料透明容器中，在光照下孵育 7 天，并每天观察。使用标准程序计算每个处理的发芽指数、发芽率、发芽时间和活力指数。鸭脚木、大托叶云实和辣木叶浸提液的有效浓度分别为 40、40 和 60%。大托叶云实、辣木和鸭脚木叶浸提液的最佳浸泡时间分别为 1、5 和 6 小时。所有叶浸提液均观察到可保护秋葵种子免受测试生物的感染。此外，与浸泡在水中的对照种子相比，浸泡在各叶浸提液中的种子表现出明显更高的发芽潜力。该研究证实，叶浸提液可能是一种有吸引力的经济替代物，可用于种子引发和保护。
