Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem, 636011, India.
Arch Microbiol. 2022 Aug 18;204(9):565. doi: 10.1007/s00203-022-03179-z.
The aim of this present work was to explore the potential feather-degrading bacterial isolates were isolated from poultry farm soil. Isolation and screening of keratinase-producing bacterial isolates were performed in keratin agar medium. The potential keratinase-producing bacterial isolates were identified using morphological, biochemical and molecular characterization. Degradation of chicken feather was optimized using different nutrient or physical factors in feather meal broth medium. Soluble peptide, amino acid and free thiol group liberation during feather degradation were estimated too. The isolated bacterial isolates were found significantly degrading the chicken feathers with keratinase enzyme production. The present study revealed a significantly novel feather-degrading Geobacillus thermodenitrificans PS41 bacterial isolate, isolated from poultry farm soil.
本研究的目的是从家禽养殖场土壤中分离潜在的羽毛降解细菌。在角蛋白琼脂培养基中进行角蛋白酶产生菌的分离和筛选。通过形态学、生化和分子特征鉴定潜在的角蛋白酶产生菌。在羽毛粉肉汤培养基中优化不同营养或物理因素来优化鸡羽毛的降解。还估计了羽毛降解过程中可溶性肽、氨基酸和游离巯基的释放。分离的细菌分离物被发现能显著降解羽毛，产生角蛋白酶。本研究从家禽养殖场土壤中分离到一株新型的羽毛降解嗜热脱硫杆菌 PS41 细菌。
