Beilke M A, Collins-Lech C, Sohnle P G
J Lab Clin Med. 1987 Jul;110(1):91-6.
Dimethyl sulfoxide (DMSO) has been demonstrated to suppress the in vitro microbicidal activity of neutrophils. In addition, this compound has been described as having significant anti-inflammatory activity. These properties have generally been attributed to the effectiveness of this compound as a hydroxyl radical scavenger. However, DMSO can also act as a reductant under certain conditions, yielding its fully oxidized form, dimethyl sulfone (DMSO2), as the product. Therefore, we evaluated the ability of these two compounds to interfere with the production of oxidants other than the hydroxyl radical by stimulated human neutrophils. In a cell-free assay, DMSO was found to quench the oxidant activity of hypochlorous acid. Neither DMSO nor DMSO2 reacted with superoxide, hydrogen peroxide, taurine chloramine, or monochloramine in this system. However, both DMSO and DMSO2 significantly suppressed the production of superoxide, hydrogen peroxide, and hypochlorous acid by human neutrophils stimulated with either phorbol myristate acetate or opsonized zymosan. Neutrophil viability was not reduced by either DMSO or DMSO2. Inhibition of the oxidative function of stimulated neutrophils by DMSO may provide an alternative explanation for the effects of this compound on the microbicidal activity of neutrophils and as an in vivo anti-inflammatory agent.
二甲基亚砜（DMSO）已被证明可抑制中性粒细胞的体外杀菌活性。此外，该化合物还被描述为具有显著的抗炎活性。这些特性通常归因于该化合物作为羟基自由基清除剂的有效性。然而，DMSO在某些条件下也可作为还原剂，生成其完全氧化形式的产物二甲基砜（DMSO2）。因此，我们评估了这两种化合物干扰受刺激的人中性粒细胞产生除羟基自由基以外的氧化剂的能力。在无细胞试验中，发现DMSO可淬灭次氯酸的氧化活性。在该系统中，DMSO和DMSO2均未与超氧化物、过氧化氢、牛磺酸氯胺或一氯胺发生反应。然而，DMSO和DMSO2均显著抑制了用佛波酯肉豆蔻酸酯或调理酵母聚糖刺激的人中性粒细胞产生超氧化物、过氧化氢和次氯酸。DMSO和DMSO2均未降低中性粒细胞的活力。DMSO对受刺激中性粒细胞氧化功能的抑制作用可能为该化合物对中性粒细胞杀菌活性的影响以及作为体内抗炎剂提供另一种解释。
