Department of Ophthalmology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Department of Pharmacy, Affiliated Hospital of Shandong University of Traditional Chinese, Jinan, China.
J Biochem Mol Toxicol. 2022 Nov;36(11):e23197. doi: 10.1002/jbt.23197. Epub 2022 Aug 19.
Fragesin, a traditional Chinese medicine, has been shown to exert anti-inflammatory effect. The aim of this study was to figure out the possible effectiveness of the fargesin, and to invest the mechanisms by which it works in the cadmium-induced lung injury in mice. Fargesin was given 1 h before cadmium treatment for 7 days. Then, the bronchoalveolar lavage fluid (BALF) were harvested to test inflammatory cells and pro-inflammatory cytokine production. Lung histopathological changes, myeloperoxidase (MPO) activity, and aryl hydrocarbon receptor (AhR) and nuclear factor kappa B (NF-κB) activation were measured. Fargesin dose-dependently reduced inflammatory cells and pro-inflammatory cytokines in BALF, improved lung histopathological injury, and inhibited lung wet/dry ratio and MPO activity. Furthermore, fargesin inhibited cadmium-induced NF-κB activation. In addition, fargesin was found to increase AhR expression. In conclusion, fargesin attenuates cadmium-induced lung injury may be via activating AhR, which subsequently suppressing the inflammatory response.
法呢醇是一种中药，已被证明具有抗炎作用。本研究旨在探讨法呢醇的可能疗效及其在镉诱导的小鼠肺损伤中的作用机制。法呢醇在镉处理前 1 小时给予，连续 7 天。然后收集支气管肺泡灌洗液（BALF）以检测炎症细胞和促炎细胞因子的产生。测量肺组织病理学变化、髓过氧化物酶（MPO）活性、芳烃受体（AhR）和核因子 kappa B（NF-κB）的激活。法呢醇呈剂量依赖性降低 BALF 中的炎症细胞和促炎细胞因子，改善肺组织病理学损伤，抑制肺湿/干比和 MPO 活性。此外，法呢醇抑制了镉诱导的 NF-κB 激活。此外，发现法呢醇增加了 AhR 的表达。总之，法呢醇减轻镉诱导的肺损伤可能是通过激活 AhR，从而抑制炎症反应。
