Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
J Pharm Biomed Anal. 2022 Oct 25;220:114992. doi: 10.1016/j.jpba.2022.114992. Epub 2022 Aug 11.
Sweet fennel (Foeniculum vulgare Mill. var. dulce) and thyme (Zataria multiflora Boiss.) are regarded as the important supplies for pharmaceutical, food, cosmetic, and perfume industries. The major components trans-anethole and thymol are represented in fennel and thyme, respectively. The essential oils (EOs) content and the value of their related constituents should be given in strict quality control due to the storage conditions, source, and adulterations. In this study, we compared the validation of quantitative H NMR (qH NMR) method with the gas chromatography with flame ionization detection (GC-FID) to quantify the trans-anethole and thymol in fennel and thyme EOs and their related supplements. The current results showed that the quantification of trans-anethole and thymol by qH NMR method was successfully achieved from their EOs and supplements. All the validation parameters including linearity, robustness, repeatability, and stability were authenticated for thymol and trans-anethole quantification. Similar results were obtained in both qH NMR and conventional GC-FID methods. Therefore, according to the measured values, the qH NMR method was adequate to determine the constituents of the EOs, with the results being roughly comparable to those obtained by GC-FID, with the advantage of being simple, repeatable, rapid (8-10 min, while for GC-FID 55 min) and essential for quality control of commercial samples.
甜茴香（Foeniculum vulgare Mill. var. dulce）和百里香（Zataria multiflora Boiss.）被认为是制药、食品、化妆品和香水行业的重要原料。反式茴香脑和百里香酚分别是甜茴香和百里香中的主要成分。由于储存条件、来源和掺假等因素，精油（EOs）的含量及其相关成分的价值应在严格的质量控制下给出。在这项研究中，我们比较了定量核磁共振（qH NMR）方法与气相色谱-火焰离子化检测（GC-FID）的验证，以定量分析甜茴香和百里香精油及其相关补充剂中的反式茴香脑和百里香酚。目前的结果表明，qH NMR 方法成功地从甜茴香和百里香精油及其补充剂中定量了反式茴香脑和百里香酚。所有验证参数，包括线性、稳健性、重复性和稳定性，均已通过对百里香酚和反式茴香脑的定量进行了验证。qH NMR 和常规 GC-FID 方法均得到了类似的结果。因此，根据测量值，qH NMR 方法足以确定 EOs 的成分，其结果与 GC-FID 大致相当，具有简单、可重复、快速（8-10 分钟，而 GC-FID 则需要 55 分钟）的优点，对商业样品的质量控制至关重要。
