一种用于细胞和溃疡性结肠炎缺氧成像的高灵敏度近红外荧光探针。
A highly sensitive NIR fluorescence probe for hypoxia imaging in cells and ulcerative colitis.
机构信息
Institute of Optical Functional Materials for Biomedical Imaging, School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong, 271016, PR China.
Department of Gastroenterology, Medical Research Center, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, 250014, PR China.
出版信息
Talanta. 2023 Jan 15;252:123834. doi: 10.1016/j.talanta.2022.123834. Epub 2022 Aug 12.
Ulcerative colitis, a kind of inflammatory bowel disease (IBD), is caused by dysregulated immune response of intestinal bacteria. This chronic disorder can lead to a deficiency of O (hypoxia) in the colon microenvironment. Nitroreductase (NTR) is a highly expressed endogenous enzyme under hypoxia, so the detection of NTR can provide diagnostic information about ulcerative colitis. Herein, an ultrasensitive NTR-triggered fluorescence probe (WS-1-NO) is developed for hypoxia imaging in ulcerative colitis. The probe shows a significant fluorescence enhancement (45-fold) after reacting with NTR, with an extremely low detection limit of 0.096 ng/mL. Furthermore, we apply it for fluorescence imaging of hypoxia in living cells, tumors and dextran sulphate sodium (DSS)-induced ulcerative colitis mouse models. We believe that the probe may be investigated as an effective potential tool for gaining insight into the hypoxia-relevant diseases, such as cancer and ulcerative colitis.
溃疡性结肠炎是一种炎症性肠病（IBD），是由肠道细菌的免疫反应失调引起的。这种慢性疾病会导致结肠微环境缺氧（O 缺乏）。硝基还原酶（NTR）是缺氧下高度表达的内源性酶，因此 NTR 的检测可以提供关于溃疡性结肠炎的诊断信息。在此，开发了一种超灵敏的 NTR 触发荧光探针（WS-1-NO），用于溃疡性结肠炎的缺氧成像。探针与 NTR 反应后，荧光显著增强（45 倍），检测限低至 0.096ng/mL。此外，我们将其应用于活细胞、肿瘤和葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎小鼠模型中的缺氧荧光成像。我们相信，该探针可以作为一种有效的潜在工具，用于深入研究与缺氧相关的疾病，如癌症和溃疡性结肠炎。
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