Institute of Optical Functional Materials for Biomedical Imaging, School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong, 271016, PR China.
Department of Gastroenterology, Medical Research Center, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, 250014, PR China.
Talanta. 2023 Jan 15;252:123834. doi: 10.1016/j.talanta.2022.123834. Epub 2022 Aug 12.
Ulcerative colitis, a kind of inflammatory bowel disease (IBD), is caused by dysregulated immune response of intestinal bacteria. This chronic disorder can lead to a deficiency of O (hypoxia) in the colon microenvironment. Nitroreductase (NTR) is a highly expressed endogenous enzyme under hypoxia, so the detection of NTR can provide diagnostic information about ulcerative colitis. Herein, an ultrasensitive NTR-triggered fluorescence probe (WS-1-NO) is developed for hypoxia imaging in ulcerative colitis. The probe shows a significant fluorescence enhancement (45-fold) after reacting with NTR, with an extremely low detection limit of 0.096 ng/mL. Furthermore, we apply it for fluorescence imaging of hypoxia in living cells, tumors and dextran sulphate sodium (DSS)-induced ulcerative colitis mouse models. We believe that the probe may be investigated as an effective potential tool for gaining insight into the hypoxia-relevant diseases, such as cancer and ulcerative colitis.
溃疡性结肠炎是一种炎症性肠病（IBD），是由肠道细菌的免疫反应失调引起的。这种慢性疾病会导致结肠微环境缺氧（O 缺乏）。硝基还原酶（NTR）是缺氧下高度表达的内源性酶，因此 NTR 的检测可以提供关于溃疡性结肠炎的诊断信息。在此，开发了一种超灵敏的 NTR 触发荧光探针（WS-1-NO），用于溃疡性结肠炎的缺氧成像。探针与 NTR 反应后，荧光显著增强（45 倍），检测限低至 0.096ng/mL。此外，我们将其应用于活细胞、肿瘤和葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎小鼠模型中的缺氧荧光成像。我们相信，该探针可以作为一种有效的潜在工具，用于深入研究与缺氧相关的疾病，如癌症和溃疡性结肠炎。
