Environmental Biotechnology and Genomics Division, CSIR-National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Environmental Biotechnology and Genomics Division, CSIR-National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440020, India.
J Environ Manage. 2022 Nov 1;321:115856. doi: 10.1016/j.jenvman.2022.115856. Epub 2022 Aug 17.
Microbes play a major role in phosphate cycling and regulate its availability in various environments. The metagenomic study highlights the microbial community divergence and interplay of phosphate metabolism functional genes in response to phosphate rich (100 mgL), limiting (25 mgL), and stressed (5 mgL) conditions at lab-scale bioreactor. Total five core phyla were found responsive toward different phosphate (Pi) levels. However, major variations were observed in Proteobacteria and Actinobacteria with 33-81% and 5-56% relative abundance, respectively. Canonical correspondence analysis reflects the colonization of Sinorhizobium (0.8-4%), Mesorhizobium (1-4%), Rhizobium (0.5-3%) in rich condition whereas, Pseudomonas (1-2%), Rhodococcus (0.2-2%), Flavobacterium (0.2-1%) and Streptomyces (0.3-4%) colonized in limiting and stress condition. The functional profiling demonstrates that Pi limiting and stress condition subjected biomass were characterized by abundant PQQ-Glucose dehydrogenase, alkaline phosphatase, 5'-nucleotidase, and phospholipases C genes. The finding implies that the major abundant genera belonging to phosphate solubilization enriched in limiting/stressed conditions decide the functional turnover by modulating the metabolic flexibility for Pi cycling. The study gives a better insight into intrinsic ecological responsiveness mediated by microbial communities in different Pi conditions that would help to design the microbiome according to the soil phosphate condition. Furthermore, this information assists in sustainably maintaining the ecological balance by omitting excessive chemical fertilizers and eutrophication.
微生物在磷循环中起着重要作用，并调节各种环境中磷的可用性。这项宏基因组研究强调了微生物群落的差异以及对富磷（100mg/L）、限制磷（25mg/L）和胁迫（5mg/L）条件下磷酸盐代谢功能基因的相互作用，这是在实验室规模的生物反应器中进行的。总共发现了五个核心门对不同的磷（Pi）水平有反应。然而，在变形菌门和放线菌门中观察到了主要的变化，相对丰度分别为 33-81%和 5-56%。典范对应分析反映了在富磷条件下Sinorhizobium（0.8-4%）、Mesorhizobium（1-4%）和 Rhizobium（0.5-3%）的定植，而在限制和胁迫条件下，Pseudomonas（1-2%）、Rhodococcus（0.2-2%）、Flavobacterium（0.2-1%）和 Streptomyces（0.3-4%）定植。功能分析表明，Pi 限制和胁迫条件下的生物量特征是丰富的 PQQ-葡萄糖脱氢酶、碱性磷酸酶、5'-核苷酸酶和磷脂酶 C 基因。这一发现表明，属于磷酸盐溶解的主要丰富属在限制/胁迫条件下富集，通过调节磷循环的代谢灵活性来决定功能周转率。该研究深入了解了微生物群落在不同 Pi 条件下介导的内在生态响应，这有助于根据土壤磷条件设计微生物组。此外，通过省略过量的化肥和富营养化，可以帮助维持生态平衡。
