Ayers L S, Typpo J T, Krause G F
J Nutr. 1987 Jun;117(6):1098-101. doi: 10.1093/jn/117.6.1098.
The isoleucine requirement of young, growing male guinea pigs was investigated by use of crystalline amino acid diets containing 3.65% nitrogen. Three-week-old guinea pigs were fed one of eight crystalline amino acid diets ranging from 0.2 to 1.2% isoleucine for 22 d. Diets were evaluated on the basis of body weight changes, nitrogen retention, carcass weight, liver weight, hematocrit, hemoglobin and carcass and liver weights expressed as percentages of live body weight. A 0.5% level of dietary isoleucine (2.2% of total nitrogen X 6.25) was the lowest level fed that did not have a response significantly lower than the higher levels fed, and that generally promoted a thrifty and well-groomed appearance of the animals.
通过使用含3.65%氮的结晶氨基酸日粮，对生长中的年轻雄性豚鼠的异亮氨酸需求进行了研究。给三周龄的豚鼠喂食八种结晶氨基酸日粮中的一种，异亮氨酸含量从0.2%到1.2%，持续22天。根据体重变化、氮潴留、胴体重、肝脏重量、血细胞比容、血红蛋白以及胴体和肝脏重量占活体体重的百分比来评估日粮。日粮中异亮氨酸水平为0.5%（总氮的2.2%×6.25）是所喂食的最低水平，该水平下的反应并不显著低于较高水平，并且通常能使动物呈现出节俭且状态良好的外观。
