Research Center in Morphology and Applied Immunology, Faculty of Medicine, University of Brasilia, Brasilia, Brazil.
Anal Biochem. 2022 Oct 15;655:114839. doi: 10.1016/j.ab.2022.114839. Epub 2022 Aug 18.
The color shift caused by the interaction between proteins and Coomassie Brilliant Blue in the Bradford assay can be recorded with a smartphone camera. Color data can then be extracted from pictures and processed as analytical signals. Here, I present a simple procedure to accurately measure protein levels using color data from pictures of microplates. Plotting the ratio of blue to green intensity (RGB scale) as a function of protein concentration results in a linear (R ≥ 0.99) relationship, from which protein levels in biological samples can be calculated with no significant difference from values obtained using absorbance data (RGB versus absorbance curves).
考马斯亮蓝与蛋白质相互作用引起的颜色位移可以用智能手机摄像头记录下来。然后可以从图像中提取颜色数据并作为分析信号进行处理。在这里，我提出了一种简单的方法，使用微孔板图像的颜色数据准确测量蛋白质水平。绘制蓝色与绿色强度的比值（RGB 比例）作为蛋白质浓度的函数，得到线性关系（R≥0.99），由此可以计算生物样品中的蛋白质水平，与使用吸光度数据（RGB 与吸光度曲线）获得的值没有显著差异。
