College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, PR China.
College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, PR China; Institute of Reproduction and Metabolism, Yangzhou University, Yangzhou, 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, PR China.
Biochem Biophys Res Commun. 2022 Oct 20;626:129-134. doi: 10.1016/j.bbrc.2022.07.084. Epub 2022 Aug 6.
Taurine, acting as a free amino acid, is widely distributed and plays multiple functions, including its regulating effect on estrogen synthesis in ovary. However, the mechanisms of taurine regulating estrogen synthesis in granulosa cells are not well understood. In this study, we identify whether microRNA-7a2 (miR-7a2) is involved in the signaling of taurine regulating estrogen synthesis in mouse granulosa cells for the first time. The results demonstrated that taurine transporter (TauT) co-localized with miR-7a in mouse ovarian granulose cells. Further, taurine treatment markedly enhanced the expression of miR-7a and Cyp19a1 in mouse ovaries and increased serum 17β-estradiol (E) concentration. Meanwhile, miR-7a2 knockout reversed the effect of taurine on E. In addition, Golgi apparatus protein 1 (Glg1), a downstream target gene of miR-7a2, was significantly down-regulated by taurine, while Glg1 knockdown markedly increased the Cyp19a1 expression and E synthesis. Moreover, taurine affected miR-7a expression via activating p38 signaling. These results suggest that taurine promotes E synthesis through p38/miR-7a/Glg1/Cyp19a1 signaling pathway, which is crucial to understand the function and mechanism of taurine on estrogen synthesis.
牛磺酸作为一种游离氨基酸，广泛分布并发挥多种功能，包括对卵巢中雌激素合成的调节作用。然而，牛磺酸调节颗粒细胞中雌激素合成的机制尚不清楚。在这项研究中，我们首次确定了是否微小 RNA-7a2（miR-7a2）参与了牛磺酸调节小鼠颗粒细胞雌激素合成的信号通路。结果表明，牛磺酸转运蛋白（TauT）与小鼠卵巢颗粒细胞中的 miR-7a 共定位。此外，牛磺酸处理显著增强了小鼠卵巢中 miR-7a 和 Cyp19a1 的表达，并增加了血清 17β-雌二醇（E）浓度。同时，miR-7a2 敲除逆转了牛磺酸对 E 的作用。此外，miR-7a2 的下游靶基因高尔基器蛋白 1（Glg1）被牛磺酸显著下调，而 Glg1 敲低显著增加了 Cyp19a1 的表达和 E 的合成。此外，牛磺酸通过激活 p38 信号通路影响 miR-7a 的表达。这些结果表明，牛磺酸通过 p38/miR-7a/Glg1/Cyp19a1 信号通路促进 E 的合成，这对于理解牛磺酸对雌激素合成的功能和机制至关重要。
