College of Pharmacy, Shanxi Medical University, Taiyuan, PR China.
Translational Medicine Research Center, Shanxi Medical University, Taiyuan, PR China.
Anal Biochem. 2022 Oct 15;655:114869. doi: 10.1016/j.ab.2022.114869. Epub 2022 Aug 19.
A novel, simple and efficient capillary electrophoresis method was developed to simultaneous determination of six furanocoumarins (psoralen, isopsoralen, imperatorin, isoimperatorin, phellopterin, and cnidilin). The separation buffer consisted of 30 mM boric acid, 12 mM sulfobutylether-β-cyclodextrin and 1.5 mM 2-hydroxypropyl-β-cyclodextrin (pH 7.8); the voltage was 20 kV, the temperature was 25 °C and the detection wavelength was at 246 nm with a diode array detector (DAD). Under the above conditions, the analytes could be separated with high resolution in less than 7 min. This method was used to simultaneously determine the content of psoralen, imperatorin, isoimperatorin and phellopterin in Angelica Dahurica Radix. And good linearities were obtained with correlation coefficients from 0.9992 to 0.9999. The limits of detection (LOD, S/N = 3) and the limits of quantitation (LOQ, S/N = 10) ranged from 0.6 to 3.0 μg/mL and from 2.1 to 9.9 μg/mL, respectively. The recoveries ranged between 98.8% and 101.8%. The results indicated the method can achieve baseline separation and quantitative analysis of furanocoumarins in Chinese herbal medicines and formulations.
建立了一种新颖、简单、高效的毛细管电泳法，用于同时测定 6 种呋喃香豆素（补骨脂素、异补骨脂素、花椒毒素、异花椒毒素、佛手柑内酯和花椒毒素）。分离缓冲液由 30 mM 硼酸、12 mM 磺丁基醚-β-环糊精和 1.5 mM 2-羟丙基-β-环糊精（pH 7.8）组成；电压为 20 kV，温度为 25°C，二极管阵列检测器（DAD）检测波长为 246 nm。在上述条件下，分析物可在 7 分钟内实现高分辨率分离。该方法用于同时测定白芷中补骨脂素、花椒毒素、异花椒毒素和佛手柑内酯的含量。得到的相关系数在 0.9992 到 0.9999 之间，具有良好的线性关系。检测限（LOD，S/N=3）和定量限（LOQ，S/N=10）的范围分别为 0.6 至 3.0 μg/mL 和 2.1 至 9.9 μg/mL。回收率在 98.8%至 101.8%之间。结果表明，该方法可用于中草药及其制剂中呋喃香豆素的基线分离和定量分析。
