Guo Jing, Wang Mengli, Guo Haitao, Chang Ruimiao, Yu Haixia, Zhang Guangbin, Chen Anjia
School of Pharmacy, Shanxi Medical University, Taiyuan, P. R. China.
Translational Medicine Research Center, Shanxi Medical University, Taiyuan, P. R. China.
Biomed Chromatogr. 2019 Nov;33(11):e4646. doi: 10.1002/bmc.4646. Epub 2019 Aug 5.
A simple, comprehensive and efficient capillary electrophoresis method using a dual cyclodextrin system was developed for the simultaneous determination of seven isoflavones (3'-methoxypuerarin, puerarin, 3'-hydroxypuerarin, ononin, daidzin, daidzein and genistin). Baseline separations of the seven isoflavones were achieved within 11 min with the running buffer consisting of 35 mm sodium tetraborate, 9.0 mm sulfobutylether-β-cyclodextrin and 30 mm α-cyclodextrin at pH 9.34, and peaks were detected at 254 nm. Other separation parameters included the separation voltage for 15 kV and the working temperature for 25°C. Under the optimum conditions, good linearities were obtained with linear correlation coefficients of seven isoflavones of 0.9978-0.9992. The limits of detection and the limits of quantification were 0.7-2.9 and 2.5-9.5 μg/mL, respectively. Excellent precision and accuracy were obtained. The intraday and interday precision ranged from 0.7 to 2.0% and from 0.8 to 1.9%, respectively. The recoveries of seven analytes were from 97.7 to 103.1%. This method was successfully applied to determine the seven analytes in Radix Puerariae and its preparations.
建立了一种使用双环糊精体系的简单、全面且高效的毛细管电泳方法，用于同时测定七种异黄酮（3'-甲氧基葛根素、葛根素、3'-羟基葛根素、芒柄花苷、大豆苷、大豆苷元和染料木苷）。在由35 mM四硼酸钠、9.0 mM磺丁基醚-β-环糊精和30 mM α-环糊精组成的运行缓冲液（pH 9.34）中，11分钟内实现了七种异黄酮的基线分离，并在254 nm处检测到峰。其他分离参数包括15 kV的分离电压和25°C的工作温度。在最佳条件下，七种异黄酮的线性相关系数为0.9978 - 0.9992，线性良好。检测限和定量限分别为0.7 - 2.9 μg/mL和2.5 - 9.5 μg/mL。获得了出色的精密度和准确度。日内和日间精密度分别为0.7%至2.0%和0.8%至1.9%。七种分析物的回收率为97.7%至103.1%。该方法成功应用于测定葛根及其制剂中的七种分析物。
