Université libre de Bruxelles (ULB), École polytechnique de Bruxelles, 3BIO-BioMatter, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium.
Université libre de Bruxelles (ULB), École polytechnique de Bruxelles, 3BIO-BioMatter, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium.
Carbohydr Polym. 2022 Nov 1;295:119844. doi: 10.1016/j.carbpol.2022.119844. Epub 2022 Jul 9.
Enzyme-mediated crosslinked hydrogels as soft materials for biomedical applications have gained considerable attention. In this article, we studied the effect of tannic acid post-treatment on adhesiveness and physiochemical properties of an enzymatically crosslinked hydrogel based on chitosan and alginate. The hydrogels were soaked in TA solution at different pH (3, 5.5, 7.4, and 9) and concentrations (1, 10, 20, 30 TA wt%). Increasing the TA concentration to 30 TA wt% and pH (up to 7.4) increased the TA loading and TA release. TA post-treatment reduced the swelling ratio and degradation rate of the hydrogels due to the formation of hydrogen bonding between TA molecules, chitosan, and alginate chains resulted in higher crosslinking density. TA-reinforced hydrogels with 30 % TA (Gel-TA 30) exhibited significantly high adhesive strength (up to 18 kPa), storage modulus (40 kPa), and antioxidant activity (>96 %), antibacterial activity, and proliferation and viability of 3 T3-L1 fibroblast cells.
酶介导的交联水凝胶作为生物医学应用的软材料引起了广泛关注。在本文中，我们研究了单宁酸（TA）后处理对基于壳聚糖和海藻酸钠的酶交联水凝胶的粘附性和物理化学性质的影响。将水凝胶在不同 pH 值（3、5.5、7.4 和 9）和浓度（1、10、20、30 TA wt%）的 TA 溶液中浸泡。随着 TA 浓度（增加到 30 TA wt%）和 pH 值（最高至 7.4）的增加，TA 负载量和释放量增加。TA 后处理降低了水凝胶的溶胀率和降解率，这是由于 TA 分子、壳聚糖和海藻酸盐链之间形成氢键，导致更高的交联密度。用 30% TA（Gel-TA 30）增强的水凝胶表现出显著高的粘附强度（高达 18 kPa）、储能模量（40 kPa）和抗氧化活性（>96%）、抗菌活性以及 3T3-L1 成纤维细胞的增殖和活力。
