Jiangxi Province Key Laboratory of Drug Design and Evaluation, School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang 330013, China.
Jiangxi Province Key Laboratory of Drug Design and Evaluation, School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang 330013, China.
Int J Biol Macromol. 2024 Nov;279(Pt 4):135417. doi: 10.1016/j.ijbiomac.2024.135417. Epub 2024 Sep 10.
Gelatin (Gel) hydrogels are widely utilized in various aspects of tissue engineering, such as wound repair, due to their abundance and biocompatibility. However, their low strength and limited functionality have constrained their development and scope of application. Tannic acid (TA), a naturally occurring polyphenol found in plants and fruits, has recently garnered interest as a crosslinking, anti-inflammatory, and antioxidant agent. In this study, we fabricated novel multifunctional gelatin methacrylate/alginate-tannin (GelMA/Alg-TA) hydrogels using chemical and physical crosslinking strategies with gelatin methacrylate (GelMA), alginate (Alg), and TA as the base materials. The GelMA/Alg-TA hydrogels maintained a stable three-dimensional porous structure with appropriate water content and exhibited excellent biocompatibility. Additionally, these hydrogels demonstrated significant antioxidant and antibacterial properties and substantially promoted wound healing in a mouse model of full-thickness skin defects by modulating inflammatory responses and enhancing granulation formation. Therefore, our study offers valuable insights into the design principles of novel multifunctional GelMA/Alg-TA hydrogels, highlighting their exceptional biocompatibility, antioxidant, and antibacterial properties. GelMA/Alg-TA hydrogels are promising candidates for wound healing applications.
明胶（Gel）水凝胶由于其丰富的来源和生物相容性而被广泛应用于组织工程的各个领域，如伤口修复。然而，其强度低和功能有限限制了其发展和应用范围。单宁酸（TA）是一种存在于植物和水果中的天然多酚，最近作为交联剂、抗炎剂和抗氧化剂受到关注。在这项研究中，我们使用化学和物理交联策略，以甲基丙烯酰化明胶（GelMA）、海藻酸钠（Alg）和 TA 为基础材料，制备了新型多功能明胶甲基丙烯酰/海藻酸盐-单宁（GelMA/Alg-TA）水凝胶。GelMA/Alg-TA 水凝胶保持了稳定的三维多孔结构，具有适当的含水量，并表现出优异的生物相容性。此外，这些水凝胶表现出显著的抗氧化和抗菌性能，并通过调节炎症反应和促进肉芽形成，显著促进了全层皮肤缺陷小鼠模型的伤口愈合。因此，我们的研究为新型多功能 GelMA/Alg-TA 水凝胶的设计原则提供了有价值的见解，突出了其出色的生物相容性、抗氧化和抗菌性能。GelMA/Alg-TA 水凝胶是伤口愈合应用的有前途的候选材料。
