Department of Bioengineering, Hanyang University, Seoul 04763, Republic of Korea.
Department of Bioengineering, Hanyang University, Seoul 04763, Republic of Korea; Institute of Nano Science and Technology, Hanyang University, Seoul 04763, Republic of Korea.
Carbohydr Polym. 2022 Nov 1;295:119846. doi: 10.1016/j.carbpol.2022.119846. Epub 2022 Jul 9.
Hydrogels have been widely exploited as inks for three-dimensional (3D) bioprinting, a useful technique for building complex biological structures with living cells. However, hydrogels have inherently limited mechanical properties (e.g., brittleness) and printability. Thus, we hypothesized that hyaluronate-based hydrogels with stretchable and self-healing properties would be useful for 3D bioprinting. Oxidized hyaluronate (oHA) and hydrazide-modified hyaluronate (hHA) formed stretchable and flexible hydrogels because of double network formation via chemical cross-linking (i.e., acylhydrazone bond formation) and physical cross-linking (i.e., charge interaction). The addition of adipic acid dihydrazide (ADH) to oHA/hHA hydrogels enhanced the self-healing capability of the gels, which were useful for fabricating 3D constructs with various shapes maintaining their stretchability even after 3D printing (about two times its original length). ATDC5 cells were viable within the 3D-printed constructs in vitro. This hydrogel system, consisting of hyaluronic acid (HA)-based polymers, may have potential for many tissue engineering applications via 3D bioprinting.
水凝胶已被广泛用作三维（3D）生物打印的墨水，这是一种构建具有活细胞的复杂生物结构的有用技术。然而，水凝胶具有固有的有限的机械性能（例如脆性）和可打印性。因此，我们假设具有拉伸性和自修复性能的基于透明质酸的水凝胶将有助于 3D 生物打印。由于通过化学交联（即酰腙键形成）和物理交联（即电荷相互作用）形成双网络，氧化透明质酸（oHA）和酰肼改性透明质酸（hHA）形成了具有拉伸性和柔韧性的水凝胶。向 oHA/hHA 水凝胶中添加己二酸二酰肼（ADH）可增强凝胶的自修复能力，这对于制造具有各种形状的 3D 结构很有用，即使在 3D 打印后（大约是其原始长度的两倍），这些结构仍保持其拉伸性。在体外，ADTC5 细胞在 3D 打印结构内具有活力。这种由透明质酸（HA）基聚合物组成的水凝胶系统，通过 3D 生物打印，可能具有许多组织工程应用的潜力。
