Pogany G C
J Reprod Fertil. 1987 May;80(1):1-12. doi: 10.1530/jrf.0.0800001.
Exposure of male mice to 6 Gy of X-rays resulted in a very rapid and extensive sloughing of the germinal epithelium as shown by the accumulation of non-sperm cells within the lumen of the epididymis. These cells were identified as stage 1 and 2 round spermatids. After accumulating in the caput, they progressed through the epididymis over the weeks of sampling and, by Week 9 after irradiation, they had completely disappeared from the organ. It is suggested that the precocious loss of round spermatids is responsible for the induction of oligospermy within the testis and the caput epididymidis. Similar sperm losses from the cauda epididymidis were not observed. Radiation also enhanced the frequency of misshapen spermatozoa normally found in this strain. From kinetic considerations, it is suggested that the generation of abnormal spermatozoa may be biphasic with an early component comprising maturing spermatids and a late contingent composed of affected spermatocytes. Return to the pre-irradiation level of abnormal frequency was not observed within the time frame of this study (10 weeks), perhaps indicating residual damage. The synchrony that existed among the various organs in terms of both sperm loss and the generation of abnormal spermatozoa may be the result of a rapid dispersion of gametes from the testis and not due to local responses as would be expected if sperm flow were affected by the irradiation. The distribution of abnormal sperm types was different in the testis from that in the epididymis, presumably because of a testicular spermatophagic mechanism specific for the removal of certain deformities. It is concluded that the kinetics of spermatogenesis, of spermiogenesis, and of sperm transport in the mouse is not affected by exposure to 6 Gy of X-rays.
将雄性小鼠暴露于6 Gy的X射线下，导致生精上皮迅速广泛脱落，附睾管腔内出现非精子细胞聚集即可证明这一点。这些细胞被鉴定为1期和2期圆形精子细胞。它们在附睾头积聚后，在采样的数周内通过附睾前进，到照射后第9周，它们已从该器官中完全消失。有人认为，圆形精子细胞的过早丢失是睾丸和附睾头少精子症形成的原因。在附睾尾未观察到类似的精子损失。辐射还增加了该品系中通常出现的畸形精子的频率。从动力学角度考虑，有人认为异常精子的产生可能是双相的，早期部分包括成熟的精子细胞，后期部分由受影响的精母细胞组成。在本研究的时间范围内（10周）未观察到异常频率恢复到照射前水平，这可能表明存在残留损伤。在精子损失和异常精子产生方面，各器官之间存在的同步性可能是由于配子从睾丸迅速分散，而不是由于精子流动受照射影响时预期的局部反应。睾丸中异常精子类型的分布与附睾中的不同，推测是由于睾丸存在一种特异性清除某些畸形的精子吞噬机制。得出的结论是，小鼠精子发生、精子形成和精子运输的动力学不受6 Gy X射线照射的影响。
