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易肥胖和抗肥胖小鼠棕色脂肪组织中的长链非编码RNA和信使核糖核酸表达谱

LncRNA and mRNA expression profiles in brown adipose tissue of obesity-prone and obesity-resistant mice.

作者信息

Wang Congcong, Duan Meng, Lin Jinhua, Wang Guowei, Gao He, Yan Mengsha, Chen Lin, He Jialing, Liu Wei, Yang Fei, Zhu Shankuan

机构信息

Chronic Disease Research Institute, The Children's Hospital, and National Clinical Research Center for Child Health, School of Public Health, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China.

Department of Nutrition and Food Hygiene, School of Public Health, School of Medicine, Zhejiang University, 866 Yu-hang-tang Road, Hangzhou, Zhejiang 310058, China.

出版信息

iScience. 2022 Jul 20;25(8):104809. doi: 10.1016/j.isci.2022.104809. eCollection 2022 Aug 19.

DOI:10.1016/j.isci.2022.104809

PMID:35992072

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9382264/

Abstract

Obesity-prone or obesity-resistant phenotypes can exist in individuals who consume the same diet type. Brown adipose tissue functions to dissipate energy in response to cold exposure or overfeeding. Long noncoding RNAs play important roles in a wide range of biological processes. However, systematic examination of lncRNAs in phenotypically divergent mice has not yet been reported. Here, the lncRNA expression profiles in BAT of HFD-induced C57BL/6J mice were investigated by high-throughput RNA sequencing. Genes that play roles in thermogenesis and related pathways were identified. We found lncRNA (Gm44502) may play a thermogenic role in obesity resistance by interacting with six mRNAs. Our results also indicated that seven differentially expressed lncRNAs (4930528G23Rik, Gm39490, Gm5627, Gm15551, Gm16083, Gm36860, Gm42002) may play roles in reducing heat production in obesity susceptibility by interacting with seven differentially expressed mRNAs. The screened lncRNAs may participate in the pathogenesis of weight regulation and provide insight into obesity therapy.

摘要

在食用相同饮食类型的个体中，可能存在易肥胖或抗肥胖的表型。棕色脂肪组织的功能是在暴露于寒冷或过度喂养时消耗能量。长链非编码RNA在广泛的生物过程中发挥重要作用。然而，尚未有关于对表型不同的小鼠中的长链非编码RNA进行系统研究的报道。在此，通过高通量RNA测序研究了高脂饮食诱导的C57BL/6J小鼠棕色脂肪组织中的长链非编码RNA表达谱。鉴定了在产热及相关途径中起作用的基因。我们发现长链非编码RNA（Gm44502）可能通过与六种信使核糖核酸相互作用在抗肥胖中发挥产热作用。我们的结果还表明，七种差异表达的长链非编码RNA（4930528G23Rik、Gm39490、Gm5627、Gm15551、Gm16083、Gm36860、Gm42002）可能通过与七种差异表达的信使核糖核酸相互作用在肥胖易感性中发挥减少产热的作用。筛选出的长链非编码RNA可能参与体重调节的发病机制，并为肥胖治疗提供思路。