Engelhard Christoph Andreas, Huang Chien, Khani Sajjad, Kasparek Petr, Prochazka Jan, Rozman Jan, Reguera David Pajuelo, Sedlacek Radislav, Kornfeld Jan-Wilhelm
Department for Biochemistry and Molecular Biology (BMB), University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark.
Laboratory of Animal Physiology, Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
Noncoding RNA. 2022 May 6;8(3):32. doi: 10.3390/ncrna8030032.
Cold and nutrient-activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via the uncoupled respiration and secretion of endocrine factors, thereby protecting mice against diet-induced obesity and improving insulin response and glucose tolerance in men. Long non-coding RNAs (lncRNAs) have recently been identified as fine-tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to interscapular brown adipose tissue (iBAT) function in a high fat diet (HFD) and cold stressed mice. We focused on , which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis, and downregulated by β-adrenergic activation in mature adipocytes. Although we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of .
寒冷和营养激活的棕色脂肪组织（BAT）能够通过解偶联呼吸和内分泌因子的分泌来增加全身能量消耗，从而保护小鼠免受饮食诱导的肥胖，并改善男性的胰岛素反应和葡萄糖耐量。长链非编码RNA（lncRNAs）最近被确定为细胞功能的微调调节因子。虽然某些lncRNAs在脂肪组织中的功能已得到表征，但其在BAT激活中的总体作用仍不清楚。我们在高脂饮食（HFD）和冷应激小鼠中鉴定了与肩胛间棕色脂肪组织（iBAT）功能相关的lncRNAs。我们重点研究了 ，它具有脂肪组织特异性表达谱，在脂肪生成过程中高度上调，在成熟脂肪细胞中被β-肾上腺素能激活下调。尽管我们在体外和体内进行了全面的转录和脂肪细胞生理学分析，但我们未检测到 功能获得或丧失的影响。
